caa a22 4500 475778510 CHVBK 20180406123637.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004649900008 doi (NATIONALLICENCE)springer-10.1007/s004649900008 Influence of CO2 pneumoperitoneum during laparoscopic surgery on cancer cell growth [Elektronische Daten] [S. Takiguchi, N. Matsuura, Y. Hamada, E. Taniguchi, M. Sekimoto, M. Tsujinaka, H. Shiozaki, M. Monden, S. Ohashi] Background: CO2 pneumoperitoneum provides a new surgical environment to treat malignant disease. The purpose of this study was to investigate the influence of CO2 pneumoperitoneum during laparoscopic surgery on cancer cell growth. Methods: WiDr human colon cancer cells were incubated for 3 h under the following two conditions: 100% CO2 at 10 mmHg, and 95% air/5% CO2 (control). Cell proliferation was assessed by the WST-1 assay and BrdU assay. Tumor growth was assessed by subcutaneous injection into 20 nude mice. Cellular damage was measured by lactate dehydrogenase (LDH) assay. Results: The number of WiDr cells under pneumoperitoneal conditions decreased in the first 24 h. However, no significant difference was observed in the proliferation rate and tumor growth of the viable cells. LDH release of the CO2 pneumoperitoneal group was higher than that of the controls. Conclusions: Our data indicate that CO2 pneumoperitoneum does not promote cancer cell proliferation but instead has a toxic effect on cancer cells. Springer-Verlag, 2000 CO2 pneumoperitoneum nationallicence Laparoscopic surgery nationallicence Cancer nationallicence Proliferation nationallicence Takiguchi S. Department of Endoscopic Surgery, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Matsuura N. Department of Pathology, School of Allied Health Sciences, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Hamada Y. Department of Pathology, School of Allied Health Sciences, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Taniguchi E. Department of Endoscopic Surgery, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Sekimoto M. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Tsujinaka M. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Shiozaki H. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Monden M. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Ohashi S. Department of Endoscopic Surgery, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut Surgical Endoscopy Springer-Verlag 14/1(2000-01-01), 41-44 0930-2794 14:1<41 2000 14 464 https://doi.org/10.1007/s004649900008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004649900008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takiguchi S. Department of Endoscopic Surgery, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Matsuura N. Department of Pathology, School of Allied Health Sciences, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Hamada Y. Department of Pathology, School of Allied Health Sciences, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Taniguchi E. Department of Endoscopic Surgery, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Sekimoto M. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Tsujinaka M. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Shiozaki H. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Monden M. Department of Surgery II, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Ohashi S. Department of Endoscopic Surgery, Osaka University, School of Medicine, 2-2 Yamadaoka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/1(2000-01-01), 41-44 0930-2794 14:1<41 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer