caa a22 4500 475779215 CHVBK 20180406123639.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004640010080 doi (NATIONALLICENCE)springer-10.1007/s004640010080 The influence of laparoscopic surgery on postoperative polymorphonuclear leukocyte function [Elektronische Daten] [C. Sietses, M. J. Wiezer, Q. A. J. Eijsbouts, P. A. M. van Leeuwen, R. H. J. Beelen, S. Meijer, M. A. Cuesta] Background: Laparoscopic surgery is thought to result in a better preservation of patients' immunological defenses. Polymorphonuclear leukocytes (PMN) are the most important effector cells in the elimination of pathogenic microorganisms. Because little is known about their function after laparoscopic surgery, we studied PMN phagocytosis, antigen expression, and oxygen radical production. Methods: In this study, 17 patients scheduled for Nissen fundoplication were randomly assigned to undergo either a laparoscopic or conventional procedure. To study phagocytic capacity, PMN were incubated with fluorescein isothiocyanate (FITC)-labeled Staphylococcus aureus. Plasma opsonic capacity was measured by comparing PMN phagocytosis in the presence of patients' own plasma with phagocytosis in the presence of control plasma. Cellular activation was measured by the expression of various cell surface markers and by assessment of PMA-stimulated oxidative burst. Results: Phagocytosis by PMN in the presence of patients' plasma was significantly lower 2 h after the conventional operation. No decrease in phagocytosis was observed when control plasma was used, indicating a decreased opsonic capacity of plasma after conventional surgery. No changes were observed after laparoscopic surgery. Furthermore, CD11b expression was significantly lower after the laparoscopic approach, indicating a blunted cellular activation. A significantly lower PMA-stimulated oxidative burst further confirmed the tempered stimulation after laparoscopic surgery. Conclusions: Laparoscopic surgery results in a preservation of the plasma opsonic capacity, and thereby the ability of PMN to phagocytose bacteria. Moreover, the postoperative cellular activation is reduced. The preserved phagocytosis and the blunted activation may prevent the development of postoperative infectious complications. Springer-Verlag New York Inc., 2000 Key words: CD11b — Laparoscopic surgery — Leukocyte — Oxidative burst — Phagocytosis nationallicence Sietses C. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut Wiezer M. J. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut Eijsbouts Q. A. J. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut van Leeuwen P. A. M. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut Beelen R. H. J. Department of Cell Biology and Immunology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut Meijer S. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut Cuesta M. A. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut Surgical Endoscopy Springer-Verlag 14/9(2000-09-01), 812-816 0930-2794 14:9<812 2000 14 464 https://doi.org/10.1007/s004640010080 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640010080 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sietses C. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Wiezer M. J. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Eijsbouts Q. A. J. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- van Leeuwen P. A. M. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Beelen R. H. J. Department of Cell Biology and Immunology, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Meijer S. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Cuesta M. A. Department of Surgery, Academic Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/9(2000-09-01), 812-816 0930-2794 14:9<812 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer