caa a22 4500 475779444 CHVBK 20180406123640.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s004640000168 doi (NATIONALLICENCE)springer-10.1007/s004640000168 The effect of carbon dioxide pneumoperitoneum on free radicals [Elektronische Daten] [M. Sare, I. Yilmaz, D. Hamamci, M. Birincioglu, M. Özmen, Ö. Yesilada] Background: Carbon dioxide is usually preferred as the insufflating agent for laparoscopic surgery because it is readily available, noncombustible, and chemically stable. It is still questionable, however, if CO2 pneumoperitoneum has any effect on free radicals and lipid peroxidation. The purpose of this study was to investigate the possible effects of CO2 pneumoperitoneum on free radicals and lipid peroxidation in the erythrocytes of rats. Methods: Fifty male Sprague-Dawley rats were divided into five equal groups: controls, a sham-operation group, and three groups of 5, 10, or 15 mmHg pneumoperitoneum with CO2. At the end of the procedure, blood was collected and the erythrocytes were separated from the plasma. The resultant supernatant fractions of erythrocytes were assayed for superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA). Results: SOD activities of the 5 and 10 mmHg pneumoperitoneum groups were significantly lower than those of the sham operation group. SOD activity was greater in the 15 mmHg pneumoperitoneum group than in any of the other groups, and this activity was significantly different from that seen in the 5 and 10 mmHg pneumoperitoneum groups (p < 0.05). No significant changes were observed in the CAT activities of the study groups (p > 0.05). MDA level was increased in the 5 mmHg pneumoperitoneum group; this result was statistically different from the control and 15 mmHg pneumoperitoneum groups (p < 0.05). No significant differences were found in the CAT activities for the study groups. On the other hand, the SOD activities of the 5 and 10 mmHg pneumoperitoneum groups were significantly lower than those of the sham and the 15 mmHg pneumoperitoneum group (p < 0.05 for all comparisons). Conclusions: These results indicate that CO2 pneumoperitoneum applied with 5-10 mmHg pressure increases the formation of free oxygen radicals by inhibiting SOD activity and that the accumulation of free radicals elevates the level of MDA, a metabolite of lipid peroxidation. The effect of CO2 pneumoperitoneum on free radicals and lipid peroxidation is pressure-dependent in rats. The mechanism underlying this pressure dependency is still under investigation. Springer-Verlag New York Inc., 2000 Key words: Carbon dioxide — Catalase — Laparoscopic surgery — Malondialdehyde — Superoxide dismutase nationallicence Sare M. Department of Surgery, Medical Faculty of Gazi University, Ankara, Turkey, Turkey aut Yilmaz I. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut Hamamci D. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut Birincioglu M. Department of Pharmacology, Medical Faculty of İnönü University, Malatya, Turkey, Turkey aut Özmen M. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut Yesilada Ö. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut Surgical Endoscopy Springer-Verlag 14/7(2000-07-01), 649-652 0930-2794 14:7<649 2000 14 464 https://doi.org/10.1007/s004640000168 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000168 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sare M. Department of Surgery, Medical Faculty of Gazi University, Ankara, Turkey, Turkey aut NATIONALLICENCE 700 1- Yilmaz I. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut NATIONALLICENCE 700 1- Hamamci D. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut NATIONALLICENCE 700 1- Birincioglu M. Department of Pharmacology, Medical Faculty of İnönü University, Malatya, Turkey, Turkey aut NATIONALLICENCE 700 1- Özmen M. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut NATIONALLICENCE 700 1- Yesilada Ö. Department of Biology and Chemistry, Arts and Sciences Faculty of İnönü University, Malatya, Turkey, Turkey aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/7(2000-07-01), 649-652 0930-2794 14:7<649 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer