caa a22 4500 475779703 CHVBK 20180406123640.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004640020035 doi (NATIONALLICENCE)springer-10.1007/s004640020035 Comparison of the stress response after laparoscopic and open cholecystectomy [Elektronische Daten] [M. Mendoza-Sagaon, E. J. Hanly, M. A. Talamini, M. F. Kutka, C. A. Gitzelmann, K. Herreman-Suquet, B. F. Poulose, C. N. Paidas, A. De Maio] Background: We designed a prospective controlled animal study to compare the stress response induced after laparoscopic and open cholecystectomy. Methods: Twelve female pigs (20-25 kg body weight) were anesthetized with ketamine, pentobarbital, and fentanyl. The animals were randomized into the following four groups: control (C), pneumoperitoneum with CO2 at 14-15 mmHg (P), laparoscopic cholecystectomy (LC), and open cholecystectomy (OC). The average duration of the procedure in each group was 35 min. Results: Central venous pressure, mean arterial pressure, pulmonary capillary wedge pressure, and cardiac output were monitored. Measurements were recorded when animals were anesthetized (baseline), immediately before and after surgery, and thereafter every 30 min for a maximum of 3 h. White blood cell count (WBC) was determined from blood samples taken before and after 3 h of surgery. Ultrasound-guided liver biopsies were done preoperatively and after 3 h of surgery. Total RNA was isolated from the liver biopsy specimens. Steady-state mRNA levels of β-fibrinogen (β-fib), α 1-chymotrypsin inhibitor (α1-CTI), metallothionein (MT), heat shock protein 70 (Hsp70), and polyubiquitin (Ub) were detected by Northern blot/hybridization. There were no statistical differences in the hemodynamic parameters among the groups. The number of circulating neutrophils and monocytes decreased only after LC. Expression of Hsp70 was not induced after any surgical procedure, and the mRNA levels of Ub did not change after surgery. The expression of α1-CTI and β-fib (acute phase genes) were similarly increased after LC and OC. Steady-state mRNA levels of MT were slightly increased after P and LC but not after OC. Conclusion: These data indicate that there are no significant differences between LC and OC in terms of induction of the stress response. Springer-Verlag New York Inc., 2000 Key words: Acute phase — Cholecystectomy — Gene expression — Inflammation — Laparoscopy — Stress nationallicence Mendoza-Sagaon M. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Hanly E. J. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Talamini M. A. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Kutka M. F. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Gitzelmann C. A. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Herreman-Suquet K. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Poulose B. F. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Paidas C. N. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut De Maio A. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut Surgical Endoscopy Springer-Verlag 14/12(2000-12-01), 1136-1141 0930-2794 14:12<1136 2000 14 464 https://doi.org/10.1007/s004640020035 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640020035 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mendoza-Sagaon M. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Hanly E. J. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Talamini M. A. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Kutka M. F. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Gitzelmann C. A. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Herreman-Suquet K. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Poulose B. F. Department of Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- Paidas C. N. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 700 1- De Maio A. Division of Pediatric Surgery, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA, US aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/12(2000-12-01), 1136-1141 0930-2794 14:12<1136 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer