caa a22 4500 47577972X CHVBK 20180406123641.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004640000189 doi (NATIONALLICENCE)springer-10.1007/s004640000189 Oxidative stress in lung tissue induced by CO2 pneumoperitoneum in the rat [Elektronische Daten] [M. Pross, H. U. Schulz, A. Flechsig, T. Manger, W. Halangk, W. Augustin, H. Lippert, T. Reinheckel] Background: Clinical trials have found that the pneumoperitoneum has potentially hazardous side effects. The biochemical basis of organ injury induced by pneumoperitoneum is, however, not well defined. Since oxidative stress is believed to play an important role in many pathological conditions, we set out to examine oxidative stress markers in the lung, liver, kidney, and pancreas by using a rat model of laparoscopy with CO2 pneumoperitoneum and comparing it to a group with gasless laparoscopy. Methods: Malondialdehyde (for lipid peroxidation), protein-bound carbonyls (for protein oxidation), reduced and oxidized glutathione, and the neutrophil marker myeloperoxidase were evaluated in tissue homogenates at 2 h, 6 h, and 18 h after laparoscopy. Immunoblotting was used to analyze the modification of lung proteins by 4-hydroxynonenal at 6 h. Results: Significant lipid peroxidation was found selectively in lungs at 2 h and 6 h after CO2 pneumoperitoneum. This was accompanied by a loss of glutathione but only minor protein oxidation. Further, lung proteins were clearly modified by the aldehydic product of lipid peroxidation 4-hydroxynonenal. Myeloperoxidase in lungs increased continuously up to 18 h in both experimental groups, but there were higher levels in the group with pneumoperitoneum. Conclusion: Oxidative stress is likely to contribute to the impairment of pulmonary function after laparoscopic operations using a CO2 pneumoperitoneum. Springer-Verlag New York Inc., 2000 Key words: Pneumoperitoneum — Carbon dioxide — Laparoscopic surgery — Lung — Oxidative stress — Lipid peroxidation nationallicence Pross M. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Schulz H. U. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Flechsig A. Department of Pathological Biochemistry, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Manger T. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Halangk W. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Augustin W. Department of Pathological Biochemistry, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Lippert H. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Reinheckel T. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut Surgical Endoscopy Springer-Verlag 14/12(2000-12-01), 1180-1184 0930-2794 14:12<1180 2000 14 464 https://doi.org/10.1007/s004640000189 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000189 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pross M. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Schulz H. U. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Flechsig A. Department of Pathological Biochemistry, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Manger T. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Halangk W. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Augustin W. Department of Pathological Biochemistry, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Lippert H. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 700 1- Reinheckel T. Department of Surgery, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany, DE aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/12(2000-12-01), 1180-1184 0930-2794 14:12<1180 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer