caa a22 4500 475779819 CHVBK 20180406123641.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004640010065 doi (NATIONALLICENCE)springer-10.1007/s004640010065 Laparoscopic surgery and Kupffer cell activation [Elektronische Daten] [F. J. Vittimberga, B. Nolan, R. A. Perugini, L. Spector, M. P. Callery] Background: Evidence tends to support a relative preservation of the systemic immune response with laparoscopy as compared with laparotomy. However, the role of hepatic macrophages, or Kupffer cells, in modulating this immune advantage is unknown. This study investigated the functions of Kupffer cells after either laparoscopy or laparotomy in a rat model. Methods: Rats underwent laparoscopy, laparotomy, or control operations. Kupffer cells were harvested, cultured, and stimulated with lipopolysaccharide. Culture supernatants were analyzed for tumor necrosis factor (TNF-α) and interleukin-6 (IL-6). Cytoplasmic lysates were analyzed for activation of two mitogen-activated protein kinases (MAPKs). Results: Production of TNF-α and IL-6 was similar in laparoscopy, laparotomy, and control groups. Both laparotomy and laparoscopy showed increased activation of p38 MAPK as compared with controls. Activation of ERK1/2 was decreased during laparotomy as compared with laparoscopy. Conclusions: Although cytokine production was similar in the laparoscopy and laparotomy groups, changes in MAPK activation suggest that intracellular pathways are more affected during laparotomy than during laparoscopy. Springer-Verlag New York Inc., 2000 Key words: Interleukin-6 — Laparoscopy — Macrophages — Signal transduction — TNF-α nationallicence Vittimberga F. J. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut Nolan B. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut Perugini R. A. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut Spector L. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut Callery M. P. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut Surgical Endoscopy Springer-Verlag 14/12(2000-12-01), 1171-1176 0930-2794 14:12<1171 2000 14 464 https://doi.org/10.1007/s004640010065 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640010065 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vittimberga F. J. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut NATIONALLICENCE 700 1- Nolan B. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut NATIONALLICENCE 700 1- Perugini R. A. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut NATIONALLICENCE 700 1- Spector L. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut NATIONALLICENCE 700 1- Callery M. P. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA, US aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/12(2000-12-01), 1171-1176 0930-2794 14:12<1171 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer