caa a22 4500 475781066 CHVBK 20180406123644.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004640000159 doi (NATIONALLICENCE)springer-10.1007/s004640000159 Hepatic tumor spread of colorectal cancer in a laparoscopic animal model [Elektronische Daten] [C. N. Gutt, Z. G. Kim, T. Gessmann, M. Lorenz, V. Paolucci] Background: We devised a standardized animal model to study the impact of laparoscopic colorectal surgery on intrahepatic tumor cell growth. Methods: The technique of laparoscopic surgery in the rat was extended by endoscopic inoculation of colon cancer cells (CC531) into the portal vein (1 × 104, 5 × 104, 1 × 105, 3 × 105, 5 × 105 cells/ml) of WAG/Rij rats (n= 25). As controls, five animals underwent laparotomy and open intraportal inoculation of 5 × 104 cells/ml. Results: Hepatic tumor growth occurred after inoculation of 5 × 104, 1 × 105, 3 × 105, and 5 × 105 cells/ml. Extrahepatic tumor and conflating hepatic tumor was observed after the inoculation of 1 × 105, 3 × 105, and 5 × 105 cells/ml. Concentrations of 5 × 104 cells/ml injected either laparoscopically or via an open technique led to single hepatic tumor nodules. No tumor growth was seen after inoculation of 1 × 104 cells/ml. Conclusions: Laparoscopic intraportal tumor cell inoculation is a feasible technique to create hepatic metastases. The inoculation of 5 × 104 CC531 cells leads to reliable cell growth that can be used to investigate the impact of various laparoscopic techniques on tumor spread. Springer-Verlag New York Inc., 2000 Key words: Laparoscopy — Colorectal surgery — Intrahepatic tumor cells — Hepatic metastases — Cancer — Animal model — Liver nationallicence Gutt C. N. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut Kim Z. G. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut Gessmann T. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut Lorenz M. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut Paolucci V. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut Surgical Endoscopy Springer-Verlag 14/5(2000-05-01), 448-451 0930-2794 14:5<448 2000 14 464 https://doi.org/10.1007/s004640000159 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000159 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gutt C. N. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut NATIONALLICENCE 700 1- Kim Z. G. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut NATIONALLICENCE 700 1- Gessmann T. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut NATIONALLICENCE 700 1- Lorenz M. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut NATIONALLICENCE 700 1- Paolucci V. Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany, DE aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/5(2000-05-01), 448-451 0930-2794 14:5<448 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer