caa a22 4500 475781198 CHVBK 20180406123644.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004640000157 doi (NATIONALLICENCE)springer-10.1007/s004640000157 The effect of immune enhancement and suppression on the development of laparoscopic port site metastasesrid=""id=""Presented at the 6th World Congress of Endoscopic Surgery, Rome, Italy, June 1998 [Elektronische Daten] [S. J. Neuhaus, D. I. Watson, T. Ellis, A. M. Rofe, G. G. Jamieson] Background: Recent clinical case reports and experimental studies have suggested that laparoscopic cancer surgery is associated with an increased risk of tumor spread to abdominal wall wounds. While the etiology of this problem was initially believed to be related to mechanical contamination of wounds, it is now recognized that there are other contributory factors, including disturbed immune function within the peritoneal cavity. To investigate this question further, we evaluated the effect of immune modulation within an established laparoscopic cancer model. Methods: Eighteen immune-competent syngeneic rats underwent modulation of their immune system, followed 18 h later by laparoscopy with the introduction of a suspension of adenocarcinoma cells into the peritoneal cavity. Rats were randomly allocated to receive either systemic cyclosporin (immune suppresser), intraperitoneal endotoxin (immune enhancer), or no agent (controls). Seven days later, all rats were killed and their peritoneal cavity was inspected for tumor implantation and port site metastases. Results: Cyclosporin did not influence the study outcome, but tumor growth (p= 0.008) and port site metastases (p < 0.0001) were less common following the administration of intraperitoneal endotoxin. Conclusion: The results of this study suggest that the immune system plays a role in the genesis of port site metastases. A preventive role for endotoxin in patients undergoing laparoscopic cancer surgery, however, remains speculative. Springer-Verlag New York Inc., 2000 Key words: Laparoscopy — Wound — Cancer — Immune system — Metastasis — Port site metastasis — Cyclosporin — Endotoxin nationallicence Neuhaus S. J. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut Watson D. I. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut Ellis T. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut Rofe A. M. The Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia, AU aut Jamieson G. G. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut Surgical Endoscopy Springer-Verlag 14/5(2000-05-01), 439-443 0930-2794 14:5<439 2000 14 464 https://doi.org/10.1007/s004640000157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neuhaus S. J. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut NATIONALLICENCE 700 1- Watson D. I. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut NATIONALLICENCE 700 1- Ellis T. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut NATIONALLICENCE 700 1- Rofe A. M. The Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia, AU aut NATIONALLICENCE 700 1- Jamieson G. G. The Royal Adelaide Centre for Endoscopic Surgery, University of Adelaide Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000 Australia, AU aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/5(2000-05-01), 439-443 0930-2794 14:5<439 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer