caa a22 4500 475781260 CHVBK 20180406123645.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004640000161 doi (NATIONALLICENCE)springer-10.1007/s004640000161 Laparoscopic surgery preserves monocyte-mediated tumor cell killing in contrast to the conventional approach [Elektronische Daten] [C. Sietses, C. E. G. Havenith, Q. A. J. Eijsbouts, P. A. M. van Leeuwen, S. Meijer, R. H. J. Beelen, M. A. Cuesta] Background: Experimental animal research shows that immunologic defenses against tumor cells are disturbed by surgical trauma, resulting in an increased rate of tumor implantation and the growth of subsequent metastases. Minimally invasive surgery is associated with a preservation of postoperative immunologic functions and, in animal models, with decreased tumor growth. The objective was to study the influence of several surgical procedures, approached conventionally and laparoscopically, on interleukin-6 (IL-6) and monocyte-mediated cytotoxicity (MMC). Methods: Five groups of five patients each were included in this prospective study: laparoscopic cholecystectomy (minor trauma) group, Nissen fundoplication (laparoscopic and conventional as moderate trauma) groups, and sigmoid colectomy (laparoscopic and conventional as major trauma) groups. Preoperatively, 1 and 4 days after surgery, IL-6 and MMC against SW948 colon cancer cell line were determined. Results: The IL-6 levels differed significantly between the three laparoscopic procedures (p= 0.004) and increased according to the degree of trauma. There was no significant difference in MMC between the three laparoscopic procedures. However, MMC was suppressed after conventional procedures and preserved after laparoscopic procedures (p= 0.001). There was no correlation between IL-6 levels and changes in MMC. Conclusions: More extensive laparoscopic procedures induce increased levels of IL-6, reflecting higher levels of trauma. Conventional surgical procedures result in depressed MMC in the postoperative period. After laparoscopic procedures, MMC is preserved. These findings may be of importance in preventing implantation and growth of cancer cells spread by surgical manipulation. Springer-Verlag New York Inc., 2000 Key words: Cytotoxicity — Interleukin 6 — Laparoscopic surgery — Monocyte — SW 948 nationallicence Sietses C. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut Havenith C. E. G. Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands, NL aut Eijsbouts Q. A. J. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut van Leeuwen P. A. M. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut Meijer S. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut Beelen R. H. J. Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands, NL aut Cuesta M. A. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut Surgical Endoscopy Springer-Verlag 14/5(2000-05-01), 456-460 0930-2794 14:5<456 2000 14 464 https://doi.org/10.1007/s004640000161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sietses C. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- Havenith C. E. G. Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- Eijsbouts Q. A. J. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- van Leeuwen P. A. M. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- Meijer S. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- Beelen R. H. J. Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands, NL aut NATIONALLICENCE 700 1- Cuesta M. A. Department of Surgery, Academic Hospital, Vrije Universiteit, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands, NL aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/5(2000-05-01), 456-460 0930-2794 14:5<456 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer