caa a22 4500 475781449 CHVBK 20180406123645.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004649900098 doi (NATIONALLICENCE)springer-10.1007/s004649900098 Port-site metastases after CO2 laparoscopy [Elektronische Daten] Is aerosolization of tumor cells a pivotal factor? [Ph. Wittich, R. Marquet, G. Kazemier, H. Bonjer] Background: Animal experiments have shown that carbon dioxide (CO2) laparoscopy results in more port-site recurrences than gasless laparoscopy. Possible transport of aerosolized tumor cells by CO2 was investigated in rats. Methods: Abdominal cavities of 15 pairs of Wistar Agouti (WAG) rats were connected and 2 × 106 or 16 × 106 CC 531 cells were injected in the first (donor) rat of each pair. Then 10 l of CO2 were allowed to flow from the first (donor) to the second (recipient) rat. Results: No tumor was found in the recipients after injection of 2 × 106 cells in the donors. Injection of 16 × 106 cells in the donors resulted in very limited tumor growth in the recipients. Conclusions: Aerosolization of tumor cells occurs, but the number of intraperitoneal tumor cells required for metastases to occur by this mechanism is extremely high. Therefore, aerosolization of tumor cells appears not to be of major relevance in the pathogenesis of port-site metastases. Springer-Verlag New York Inc., 2000 Key words: Aerosolization — Laparoscopy — Metastases — Pneumoperitoneum — Port site — Tumor nationallicence Wittich Ph Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut Marquet R. Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut Kazemier G. Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut Bonjer H. Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut Surgical Endoscopy Springer-Verlag 14/2(2000-02-01), 189-192 0930-2794 14:2<189 2000 14 464 https://doi.org/10.1007/s004649900098 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004649900098 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wittich Ph Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Marquet R. Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Kazemier G. Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 700 1- Bonjer H. Department of Surgery, University Hospital Rotterdam-Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands, The Netherlands aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/2(2000-02-01), 189-192 0930-2794 14:2<189 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer