caa a22 4500 475781473 CHVBK 20180406123645.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004649900087 doi (NATIONALLICENCE)springer-10.1007/s004649900087 Time course of differences in lymphocyte proliferation rates after laparotomy vs CO2 insufflation [Elektronische Daten] [S. Lee, J. Southall, N. Gleason, E. Huang, M. Bessler, R. Whelan] Background: Our laboratory has previously demonstrated that cell-mediated immune function is significantly more suppressed after both laparotomy and laparoscopic-assisted bowel resection than after peritoneal insufflation or open bowel resection, as assessed by delayed-type hypersensitivity (DTH) response. The purpose of this study was to further evaluate cell-mediated immunity by examining lymphocyte proliferation rates after laparotomy vs CO2 insufflation. Methods: Female Balb/C mice (n= 75) were randomly divided into the following three groups: (a) anesthesia control (A/C), (b) CO2 insufflation (INS), and (c) sham laparotomy (OPEN). The A/C group mice underwent no procedure. The INS group underwent insufflation with CO2 gas at 4-6 mmHg for 20 min. The OPEN group underwent a midline incision from xiphoid to pubic symphysis, which was clipped closed after 20 min. Splenocytes were obtained via splenic harvest and lymphocyte isolation on postoperative days (POD) 1, 2, 3, 4, and 8. Lymphocyte proliferation was determined by a nonradioactive colorimetric MTS/PMS assay 72 h after concanavalin A stimulation. Results: The laparotomy group's lymphocyte proliferation rates were significantly lower than both the control and the insufflation groups on POD 2, POD 3, and POD 4. On POD 1 and POD 8, there were no significant differences in lymphocyte proliferation among the three groups. No differences were found between the control and insufflation groups at any point. Conclusions: After stimulation, lymphocytes proliferate at a lower rate after laparotomy than after CO2 insufflation. Significant differences in lymphocyte proliferation rates between groups persist at least through POD 4. By POD 8, the mean lymphocyte proliferation rate in the laparotomy group was back to the baseline level. Our results suggest greater immunosuppression after sham laparotomy than after CO2 insufflation. Springer-Verlag New York Inc., 2000 Key words: Laparoscopy — CO2 insufflation — Lymphocyte proliferation — Immunosuppression — Concanavalin A nationallicence Lee S. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut Southall J. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut Gleason N. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut Huang E. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut Bessler M. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut Whelan R. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut Surgical Endoscopy Springer-Verlag 14/2(2000-02-01), 145-148 0930-2794 14:2<145 2000 14 464 https://doi.org/10.1007/s004649900087 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004649900087 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee S. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut NATIONALLICENCE 700 1- Southall J. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut NATIONALLICENCE 700 1- Gleason N. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut NATIONALLICENCE 700 1- Huang E. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut NATIONALLICENCE 700 1- Bessler M. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut NATIONALLICENCE 700 1- Whelan R. Department of Surgery, Columbia-Presbyterian Medical Center, 161 Fort Washington Avenue, New York, NY 10032, USA, USA aut NATIONALLICENCE 773 0- Surgical Endoscopy Springer-Verlag 14/2(2000-02-01), 145-148 0930-2794 14:2<145 2000 14 464 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer