caa a22 4500 475781791 CHVBK 20180406123646.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004640000257 doi (NATIONALLICENCE)springer-10.1007/s004640000257 Effect of CO2 pneumoperitoneum on early cellular markers of retinal ischemia in rabbits with α-chymotrypsin-induced glaucomarid=""id=""Presented in abstract form at the annual meeting of the Société Française d'Anesthésie-Réanimation, Paris, France, September, 1999, and the annual meeting of the European Society of Anaesthesiologists, Vienna, Austria, 2000 April [Elektronische Daten] [C. Lentschener, D. Fredi-Reygrobellet, H. Bouaziz, J. X. Mazoit, F. Niessen, D. Benhamou] Background: Increased intraperitoneal pressure in the head-down position is associated with a significant increase in intraocular pressure (IOP) in rabbits with α-chymotrypsin-induced glaucoma. Also, the retinal cells are weakened by the induction of increased IOP, and/or glaucoma, even when IOP is controlled by adequate therapy; therefore, these cells need to be protected from any additional aggression. Actin and vimentin are proteins of the retinal cell cytoskeleton that react readily in response to retinal injuries, including ischemia and glaucoma. Early changes in these cytoskeleton proteins determine the morphological changes observed after retinal damage. Therefore, we set out to investigate intracytoplasmic changes in vimentin and actin after a 4-h CO2 pneumoperitoneum in the head-down position in rabbits with α-chymotrypsin-induced glaucoma. Methods: Twenty-one rabbits with α-chymotrypsin-induced glaucoma in one eye received general anesthesia for 4 h in the head-down position and were randomly allocated to have (a) no pneumoperitoneum, (b) a 10 mmHg CO2 pneumoperitoneum, or (c) a 20 mmHg CO2 pneumoperitoneum. At the end of the trial, both the right glaucomatous and the left control eyes were enucleated and investigated immunocytochemically for alterations in vimentin and actin, and morphologically for retinal layer disorganization. Results: Except for the preexisting morphological changes induced by glaucoma, both the control and the glaucomatous eyes in all rabbits appeared normal in terms of retinal layer organization and the distribution of intracellular vimentin and actin whatever the intraperitoneal pressure level applied. Conclusion: In rabbits with α-chymotrypsin-induced glaucoma, a 4-h CO2 pneumoperitoneum of ≤20 mmHg in the head-down position did not induce either retinal layer disorganization or alteration of actin or vimentin. Springer-Verlag New York Inc., 2000 Key words: Eye — Intraocular pressure — Glaucoma — Müller cells — Laparoscopy — Pneumoperitoneum — Peritoneal pressure — Retinal ischemia — Cytoskeleton — Actin — Vimentin — Rabbits nationallicence Lentschener C. Department of Anesthesia, Université Paris-Sud, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France, FR aut Fredi-Reygrobellet D. Department of Histology, Faculté de Médecine, avenue de Vallombrose, 06107 Nice Cedex 02, France, FR aut Bouaziz H. Department of Anesthesia, Hôpital Central, 54035 Nancy Cedex, France, FR aut Mazoit J. X. Anesthesiology Laboratory, Université Paris-Sud, Hôpital de Bicêtre, 78 avenue du Géneral Leclerc, 94275 le Kremlin Bicêtre Cedex, France, FR aut Niessen F. Department of Ophtalmology, Université Paris-Sud, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France, FR aut Benhamou D. Department of Anesthesia, Université Paris-Sud, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France, FR aut https://doi.org/10.1007/s004640000257 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000257 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lentschener C. Department of Anesthesia, Université Paris-Sud, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France, FR aut NATIONALLICENCE 700 1- Fredi-Reygrobellet D. Department of Histology, Faculté de Médecine, avenue de Vallombrose, 06107 Nice Cedex 02, France, FR aut NATIONALLICENCE 700 1- Bouaziz H. Department of Anesthesia, Hôpital Central, 54035 Nancy Cedex, France, FR aut NATIONALLICENCE 700 1- Mazoit J. X. Anesthesiology Laboratory, Université Paris-Sud, Hôpital de Bicêtre, 78 avenue du Géneral Leclerc, 94275 le Kremlin Bicêtre Cedex, France, FR aut NATIONALLICENCE 700 1- Niessen F. Department of Ophtalmology, Université Paris-Sud, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France, FR aut NATIONALLICENCE 700 1- Benhamou D. Department of Anesthesia, Université Paris-Sud, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer