caa a22 4500 475781805 CHVBK 20180406123646.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004640000118 doi (NATIONALLICENCE)springer-10.1007/s004640000118 Is it safe to reuse disposable laparoscopic trocars?rid="" [Elektronische Daten] An in vitro testingid=""Presented at the ELSA meeting, Shanghai, China, 1998 [A. C. W. Chan, M. Ip, A. Koehler, B. Crisp, J. S. Tam, S. C. S. Chung] Background: The reuse of disposable laparoscopic instruments carries a risk of transmitting infectious diseases such as hepatitis and HIV. We evaluated the safety of reusing disposable trocars by studying the chances of their harboring infectious viruses after resterilization in an in vitro setting. Methods: Disposable laparoscopic trocars were exposed to horse blood contaminated with high or low viral concentrations of herpes simplex virus type 1 (HSV1) and attenuated polio virus type 1 at room temperature for 2 h. HSV1 was chosen as the surrogate for lipid viruses that include hepatitis B and HIV virus; polio virus represented the nonlipid viruses that cause infections in immunocompromised patients and are more resistant to sterilization. The trocars were subsequently cleaned and resterilized by low-temperature steam and formaldehyde at 80°C for 3 h. Viral cultures were then repeated after sterilization. Results: A cytopathic effect (CPE) was demonstrated at both concentrations for HSV1 in all trocars before but not after sterilization. For the polio virus, CPE was evident in 50% of the trocars (two of four) exposed to high viral concentration after sterilization. Conclusion: Disposable trocars are difficult to resterilize and may harbor infectious viruses after their initial use. Therefore, the reuse of disposable trocars in laparoscopic surgery cannot be recommended. Springer-Verlag New York Inc., 2000 Key words: Disposable laparoscopic trocars — Resterilization — Reuse — Safety nationallicence Chan A. C. W. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut Ip M. Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut Koehler A. Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut Crisp B. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut Tam J. S. Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut Chung S. C. S. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut https://doi.org/10.1007/s004640000118 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004640000118 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chan A. C. W. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut NATIONALLICENCE 700 1- Ip M. Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut NATIONALLICENCE 700 1- Koehler A. Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut NATIONALLICENCE 700 1- Crisp B. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut NATIONALLICENCE 700 1- Tam J. S. Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut NATIONALLICENCE 700 1- Chung S. C. S. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer