caa a22 4500 475782372 CHVBK 20180406123648.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1023/A:1008925722623 doi (NATIONALLICENCE)springer-10.1023/A:1008925722623 Quantitative analysis of the regulation of leukocyte chemosensory migration by a vascular prosthetic biomaterial [Elektronische Daten] [C. Chang, S. Lieberman, P. Moghe] The need for improved, infection-resistant vascular biomaterials calls for more objective evaluation of the immune pathophysiology of implantable prosthetic materials. In this study we have developed a new strategy to quantitatively characterize population-averaged responses of immune cell migration on vascular prosthetic materials. This approach, incorporating a chemokinetically regulated "biomaterial-gel” sandwich configuration, was applied to quantify both random and directed modes of the chemosensory migration of human neutrophil leukocytes on expanded polytetrafluoroethylene (ePTFE). Our studies show that (a) microporous, synthetic materials like ePTFE suppress the basal rate of random cell migration relative to that reported on non-porous control surfaces; (b) stimulation with chemoattractant (formyl peptide) can significantly elevate rates of random and directed migration on ePTFE; and (c) protein conditioning of ePTFE with albumin or immunoglobulin G can differentially modulate the rates and relative proportion of random and directional components of leukocyte migration response to chemoattractant. This, to our knowledge, is the first objective quantitation of chemokinetically regulated cell migration on implantable prosthetic materials. ©2000 Kluwer Academic Publishers Kluwer Academic Publishers, 2000 Chang C. Department of Chemical and Biochemical Engineering, Rutgers University, 08854-8058, Piscataway, NJ, USA aut Lieberman S. Department of Chemical and Biochemical Engineering, Rutgers University, 08854-8058, Piscataway, NJ, USA aut Moghe P. Department of Chemical and Biochemical Engineering, Rutgers University, 08854-8058, Piscataway, NJ, USA aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/6(2000-06-01), 337-344 0957-4530 11:6<337 2000 11 10856 https://doi.org/10.1023/A:1008925722623 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008925722623 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang C. Department of Chemical and Biochemical Engineering, Rutgers University, 08854-8058, Piscataway, NJ, USA aut NATIONALLICENCE 700 1- Lieberman S. Department of Chemical and Biochemical Engineering, Rutgers University, 08854-8058, Piscataway, NJ, USA aut NATIONALLICENCE 700 1- Moghe P. Department of Chemical and Biochemical Engineering, Rutgers University, 08854-8058, Piscataway, NJ, USA aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/6(2000-06-01), 337-344 0957-4530 11:6<337 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer