caa a22 4500 475782402 CHVBK 20180406123648.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1023/A:1008915801187 doi (NATIONALLICENCE)springer-10.1023/A:1008915801187 PolyHEMA and polyHEMA-poly(MMA-co-AA) as substrates for culturing Vero cells [Elektronische Daten] [C. Lombello, S. Malmonge, M. Wada] Poly (2-hydroxyethyl methacrylate), polyHEMA, is known to prevent cellular attachment and spreading. This hydrogel is used to culture cells not dependent on anchorage. Blending polyHEMA with a copolymer of methyl methacrylate and acrylic acid introduces negative charges to the hydrogel and improves its mechanical characteristics. PolyHEMA and the blend were tested for attachment and proliferation of Vero cells. Dense and porous samples of the hydrogels were used. Attachment assays included cellular quantification with MTT photometry and cellular morphology with the scanning electron microscopy after 2 h culture. Proliferation assays were carried out with 5 and 10 days culture. Cellular morphology included cytochemistry of resin sections and scanning electron microscope observations. Hydrogels allowed a few cells to attach and proliferate. The cells growing on the surface of hydrogels were organized in various layers and showed a differential morphology. Cells located inside the pores remained rounded. The hydrogels showed the possibility of inducing differentiated phenotypic expression. Kluwer Academic Publishers, 2000 Lombello C. Department of Cellular Biology, Biology Institute State University of Campinas, Caixa Postal-6109, CEP-13083-970, Campinas/SP, Brasil aut Malmonge S. Department of Cellular Biology, State University of Campinas, Caixa Postal-6109, CEP-13083-970, Campinas/SP, Brasil aut Wada M. Department of Cellular Biology, Biology Institute State University of Campinas, Caixa Postal-6109, CEP-13083-970, Campinas/SP, Brasil aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/9(2000-09-01), 541-546 0957-4530 11:9<541 2000 11 10856 https://doi.org/10.1023/A:1008915801187 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008915801187 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lombello C. Department of Cellular Biology, Biology Institute State University of Campinas, Caixa Postal-6109, CEP-13083-970, Campinas/SP, Brasil aut NATIONALLICENCE 700 1- Malmonge S. Department of Cellular Biology, State University of Campinas, Caixa Postal-6109, CEP-13083-970, Campinas/SP, Brasil aut NATIONALLICENCE 700 1- Wada M. Department of Cellular Biology, Biology Institute State University of Campinas, Caixa Postal-6109, CEP-13083-970, Campinas/SP, Brasil aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/9(2000-09-01), 541-546 0957-4530 11:9<541 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer