caa a22 4500 475782410 CHVBK 20180406123648.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1023/A:1008984304821 doi (NATIONALLICENCE)springer-10.1023/A:1008984304821 Biocompatibility of microplates for culturing epithelial renal cells evaluated by a microcalorimetric technique [Elektronische Daten] [Yi Xie, J. DePierre, L. Na¨ssberger] In the present study we have developed a microcalorimetric procedure which allows convenient investigation of biocompatibility in a microsystem. We examined the biocompatibility of a porcine renal epithelial tubule cell line LLC-PK1 and a human primary renal epithelial tubule cell (RPTEC) with microplates composed of three different materials, i.e. Thermanox, transparent film and titanium. All three materials showed equal biocompatibility with LLC-PK1 cells, judging from the attainment of steady-state power curves and the same rate of heat production per cell (2.5 μW / μg DNA). The human renal cells were poorly biocompatible with the Thermanox and transparent film. However, on titanium the RPTEC cell did adhere, as demonstrated by a steady-state power curve. The human cells also showed a higher metabolic activity (3.0 μW / μg DNA), than did LLC-PK1 cells cultured on the same type of microplates. In research on biocompatibility there is a need for alternatives to experimental animal investigations. The present technique allows studies of cellular interactions with different biomaterials in a rapid and standardized manner and may therefore prove to be a useful screening procedure. Kluwer Academic Publishers, 2000 Xie Yi Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden aut DePierre J. Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden aut Na¨ssberger L. Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/9(2000-09-01), 587-591 0957-4530 11:9<587 2000 11 10856 https://doi.org/10.1023/A:1008984304821 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008984304821 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xie Yi Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden aut NATIONALLICENCE 700 1- DePierre J. Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden aut NATIONALLICENCE 700 1- Na¨ssberger L. Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/9(2000-09-01), 587-591 0957-4530 11:9<587 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer