caa a22 4500 475782674 CHVBK 20180406123649.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1023/A:1008957630020 doi (NATIONALLICENCE)springer-10.1023/A:1008957630020 Surface topography and HA filler volume effect on primary human osteoblasts in vitro [Elektronische Daten] [M. Dalby, L. Silvio, G. Davies, W. Bonfield] HAPEXTM, a bone analog material, with similar properties to cortical bone, has been studied in vitro with particular reference to the effect of surface topography. The stimulation of a favorable bone response by this composite depends on optimization of the hydroxyapatite (HA) content in relation to the material bioactivity without compromising the mechanical characteristics. In this study we have started to investigate the effects of surface topography on cell attachment and subsequent cellular behavior in relation to proliferation. Different volumes of HA (20% and 40%) were added to a high density polyethylene (HDPE) matrix to produce the test materials. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to examine cell morphology on HAPEXTM, and the surface characteristics produced by different machining protocols. The measurement of cellular DNA and tritiated thymidine ([3H]-TdR) incorporation has been used to asses cell proliferation upon the materials. The results show that the material surface topography has a large influence on cell proliferation and attachment, and with a controled material topography the 40% vol HA/HDPE composite gives the greater biological response compared to the 20% vol HA/HDPE composite. Kluwer Academic Publishers, 2000 Dalby M. IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, HA7 4LP, Middlesex, UK aut Silvio L. IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, HA7 4LP, Middlesex, UK aut Davies G. *IRC in Biomedical Materials, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Bonfield W. IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, HA7 4LP, Middlesex, UK aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/12(2000-12-01), 805-810 0957-4530 11:12<805 2000 11 10856 https://doi.org/10.1023/A:1008957630020 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008957630020 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dalby M. IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, HA7 4LP, Middlesex, UK aut NATIONALLICENCE 700 1- Silvio L. IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, HA7 4LP, Middlesex, UK aut NATIONALLICENCE 700 1- Davies G. *IRC in Biomedical Materials, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Bonfield W. IRC in Biomedical Materials, Institute of Orthopaedics, Brockley Hill, Stanmore, HA7 4LP, Middlesex, UK aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/12(2000-12-01), 805-810 0957-4530 11:12<805 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer