caa a22 4500 475782704 CHVBK 20180406123649.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1008975507654 doi (NATIONALLICENCE)springer-10.1023/A:1008975507654 Proliferation/differentiation of osteoblastic human alveolar bone cell cultures in the presence of stainless steel corrosion products [Elektronische Daten] [M. Costa, M. Fernandes] Human osteoblastic alveolar bone cells were cultured for 28 days in control conditions and in the presence of three non-lethal concentrations of AISI 316L stainless steel (SS) corrosion products. Cells were exposed to SS corrosion products in two experimental situations: (i) in selected stages of the incubation time (during the first, second, third and fourth week of culture); and (ii) during the 28 days incubation period. Cultures were characterized for cell proliferation, total protein content, alkaline phosphatase activity (ALP) and ability to form mineralized deposits; culture media was analyzed for ionized calcium (Ca) and phosphorus (P) concentrations throughout the incubation period. The presence of SS corrosion products during the different stages of the incubation period did not significantly affect the cell proliferation; however, a significant dose-dependent deleterious effect was observed on the levels and pattern of ALP activity, concentration of ionized Ca and P in the culture medium and, also, ability to form mineralized deposits, especially in cultures exposed during the first and second week of culture (respectively, lag phase and exponential cell growth phase). Similar effects were observed in cultures exposed to the SS corrosion products during the 28 days incubation period. However, the presence of such products during the third week (when the mineralization process occurs) and, also, during the fourth week, resulted in little or no significant effects on the behavior of alveolar bone cells. Results suggested that SS corrosion products above certain non-lethal concentrations may disturb the proliferation/differentiation relationship of osteoblastic human alveolar bone cell cultures. ©©2000 Kluwer Academic Publishers Kluwer Academic Publishers, 2000 Costa M. Instituto de Cie^ncias Biome´dicas Abel Salazar, Largo Abel Salazar, 4000, Porto, Portugal aut Fernandes M. Faculdade de Medicina Denta´ria da Universidade do Porto, Rua Dr Manuel Pereira da Silva, 4200, Porto, Portugal aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/3(2000-03-01), 141-153 0957-4530 11:3<141 2000 11 10856 https://doi.org/10.1023/A:1008975507654 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008975507654 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costa M. Instituto de Cie^ncias Biome´dicas Abel Salazar, Largo Abel Salazar, 4000, Porto, Portugal aut NATIONALLICENCE 700 1- Fernandes M. Faculdade de Medicina Denta´ria da Universidade do Porto, Rua Dr Manuel Pereira da Silva, 4200, Porto, Portugal aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/3(2000-03-01), 141-153 0957-4530 11:3<141 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer