caa a22 4500 475782739 CHVBK 20180406123649.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1008931725401 doi (NATIONALLICENCE)springer-10.1023/A:1008931725401 Expression of heat shock protein 70 (HSP70) at the interface of polymer-implants in vivo [Elektronische Daten] [B. Klosterhalfen, U. Klinge, L. Tietze, U. Henze, L. Muys, C. Mittermayer, R. Bhardwaj] Synthetic polymer meshes are widely applied in the modern surgical approach for repairing abdominal wall defects. The implanted material is often observed leading to post-operative complications such as deficient abdominal wall mobility and adhesion formation with the abdominal cavity and/or abdominal organs. However, the functioning of the implant is primarily affected by the wound healing process guided by inflammatory events occurring at the tissue-material interface. This could presumably be influenced by the physicochemical properties of the polymer. With regard to it, the cellular and molecular processes involved in the successful restoration of the abdominal wall function are poorly understood. The present in vivo study, therefore, exemplary investigated in a rat model, the commercially available polymer-meshes Prolene® (polypropylene, PP), Mersilene® (polyester, PE) and Vicryl® (polyglactin 910), as well as new mesh variants consisting either of PP (EB) or a combination of PP and polyglactin 910 (A plus or Vypro®). The implanted material was evaluated by light and electron microscopy, immunohistochemistry as well as morphometry over an implantation period of 90 days. The data show that polymers induce heat shock protein (HSP)70, and its expression at the interface correlates inversely with the activity of the inflammatory reaction in vivo. Further, an ascent in HSP70 expression parallels the increasing implantation period and evolving foreign-body granulomas. Accordingly, a major role for HSP70 in modulating the local acceptance of polymers and as an additional marker for in vivo testing of polymers is suggestive. ©©2000 Kluwer Academic Publishers Kluwer Academic Publishers, 2000 Klosterhalfen B. The Institutes of Pathology and IZKF BIOMAT and the Department of Surgery, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Klinge U. The Institutes of Pathology and IZKF BIOMAT and the Department of Surgery, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Tietze L. The Institutes of Pathology, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Henze U. The Institutes of IZKF BIOMAT, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Muys L. The Institutes of Pathology, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Mittermayer C. The Institutes of Pathology, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Bhardwaj R. The Institutes of IZKF BIOMAT, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/3(2000-03-01), 175-181 0957-4530 11:3<175 2000 11 10856 https://doi.org/10.1023/A:1008931725401 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008931725401 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klosterhalfen B. The Institutes of Pathology and IZKF BIOMAT and the Department of Surgery, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Klinge U. The Institutes of Pathology and IZKF BIOMAT and the Department of Surgery, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Tietze L. The Institutes of Pathology, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Henze U. The Institutes of IZKF BIOMAT, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Muys L. The Institutes of Pathology, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Mittermayer C. The Institutes of Pathology, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 700 1- Bhardwaj R. The Institutes of IZKF BIOMAT, The Technical University of Aachen, Pauwelsstrasse 30, D-52057, Aachen, Germany aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/3(2000-03-01), 175-181 0957-4530 11:3<175 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer