caa a22 4500 475782771 CHVBK 20180406123649.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008901612674 doi (NATIONALLICENCE)springer-10.1023/A:1008901612674 Development of soluble glasses for biomedical use Part II: The biological response of human osteoblast cell lines to phosphate-based soluble glasses [Elektronische Daten] [V. Salih, K. Franks, M. James, G. Hastings, J. Knowles, I. Olsen] Soluble glasses are considered to be of potential clinical value in orthopaedic and dental surgery. However, the biological response to these materials is not well understood. To determine the effects of these glasses, two human osteoblast cell lines, MG63 and HOS (TE85), were incubated in vitro in the presence of increasing concentrations of extracts of the glasses. The effects of the extracts on cell growth was measured using the MTT assay and an ELISA assay was used to measure the expression of bone sialoprotein (BSP), osteonectin (ON) and fibronectin (FN), antigens which play a fundamental part in the integrity and function of hard connective tissue. The results showed that the proliferation of the cells was adversely affected only by the more soluble glasses, which also down-regulated the expression of the bone-associated proteins. In contrast, the extract of the glass with the lowest dissolution rate, which contains relatively elevated levels of Ca2+, was found to enhance bone cell growth and antigen expression. These findings suggest that the compositions of these glasses at least partly determine the response of cells and thus, that the glasses could be modified to elicit a more optimal biological response and clinical efficacy. Kluwer Academic Publishers, 2000 Salih V. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Franks K. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut James M. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Hastings G. Institute of Materials Research and Engineering, The National University of Singapore, 119260, Kent Ridge, Singapore aut Knowles J. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Olsen I. Departments of Biomaterials and Periodontology, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/10(2000-10-01), 615-620 0957-4530 11:10<615 2000 11 10856 https://doi.org/10.1023/A:1008901612674 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008901612674 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salih V. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Franks K. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- James M. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Hastings G. Institute of Materials Research and Engineering, The National University of Singapore, 119260, Kent Ridge, Singapore aut NATIONALLICENCE 700 1- Knowles J. Departments of Biomaterials, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Olsen I. Departments of Biomaterials and Periodontology, Eastman Dental Institute, University of London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/10(2000-10-01), 615-620 0957-4530 11:10<615 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer