caa a22 4500 475782836 CHVBK 20180406123649.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008974217217 doi (NATIONALLICENCE)springer-10.1023/A:1008974217217 Adhesion and proliferation of keratinocytes on ion beam modified polyethylene [Elektronische Daten] [V. ŠVORČÍk, K. WalachovÁ, K. ProŠKOVÁ, B. DvoŘÁNKOVÁ, D. VogtovÁ, R. ÖCHSNER, H. Ryssel] Polyethylene (PE) foils were modified by irradiation with ArE + and XeE + ions to different fluences and different physico-chemical properties of the irradiated PE were studied in relation to adhesion and proliferation of keratinocytes on the modified surface. Changes in the PE surface roughness were examined using the AFM technique, the production of conjugated double bonds and oxidized structures by UV-VIS and FTIR techniques respectively. The surface polarity was determined by measuring surface contact angle and two-point technique was used for the determination of PE sheet resistance. Adhesion and proliferation of keratinocytes was characterized using the MTT-test. The ion irradiation leads to creation of conjugated double bonds which, together with progressive carbonization, contribute to the observed decrease of sheet resistance. Oxidation of the irradiated PE surface layer during the ion implantation is observed. Besides oxidation, the PE surface polarity is affected by other factors. The observed increase of the PE surface roughness due to the ion irradiation is inversely proportional to the ion size. The adhesion and proliferation of keratinocytes on the ion irradiated PE is significantly higher than on the pristine PE. Distribution of results in keratinocyte cultivation and the number of cells is related to the ion fluence applied and to ion species as well. Kluwer Academic Publishers, 2000 ŠVORČÍk V. Department of Solid State Engineering, Institute of Chemical Technology, 166 28, Prague, Czech Republic aut WalachovÁ K. Department of Solid State Engineering, Institute of Chemical Technology, 166 28, Prague, Czech Republic aut ProŠKOVÁ K. Department of Solid State Engineering, Institute of Chemical Technology, 166 28, Prague, Czech Republic aut DvoŘÁNKOVÁ B. Prague Burn Center, 3rd Faculty of Medicine, Carles University, 100 34, Prague, Czech Republic aut VogtovÁ D. Prague Burn Center, 3rd Faculty of Medicine, Carles University, 100 34, Prague, Czech Republic aut ÖCHSNER R. Fraunhofer-institut fu¨r Intergierte Schaltungen, 91058, Erlangen, Germany aut Ryssel H. Fraunhofer-institut fu¨r Intergierte Schaltungen, 91058, Erlangen, Germany aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/10(2000-10-01), 655-660 0957-4530 11:10<655 2000 11 10856 https://doi.org/10.1023/A:1008974217217 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008974217217 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- ŠVORČÍk V. Department of Solid State Engineering, Institute of Chemical Technology, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- WalachovÁ K. Department of Solid State Engineering, Institute of Chemical Technology, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- ProŠKOVÁ K. Department of Solid State Engineering, Institute of Chemical Technology, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- DvoŘÁNKOVÁ B. Prague Burn Center, 3rd Faculty of Medicine, Carles University, 100 34, Prague, Czech Republic aut NATIONALLICENCE 700 1- VogtovÁ D. Prague Burn Center, 3rd Faculty of Medicine, Carles University, 100 34, Prague, Czech Republic aut NATIONALLICENCE 700 1- ÖCHSNER R. Fraunhofer-institut fu¨r Intergierte Schaltungen, 91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Ryssel H. Fraunhofer-institut fu¨r Intergierte Schaltungen, 91058, Erlangen, Germany aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/10(2000-10-01), 655-660 0957-4530 11:10<655 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer