caa a22 4500 475782992 CHVBK 20180406123650.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1008970108339 doi (NATIONALLICENCE)springer-10.1023/A:1008970108339 A percutaneous device to study glucose kinetics in subcutaneous tissue fluid [Elektronische Daten] [M. Gerritsen, J. Lutterman, J. Jansen] In the current study subcutaneous glucose kinetics were investigated in tissue fluid collected with a percutaneous device (PD). PDs containing a subcutaneous tissue chamber were implanted subcutaneously in New Zealand white rabbits. Sintered titanium fiber mesh sheets were used for subcutaneous anchorage of the PD. The bottom of the subcutaneous tissue chamber was either covered with a titanium fiber mesh sheet, a cellulose acetate membrane, or left uncovered. Subcutaneous glucose kinetics were determined after injection of octreotide and glucagon. The tissue reaction to the implants was evaluated histologically. No dynamic relationship was observed between glycaemia and subcutaneous tissue fluid glucose for all membrane covered devices. Histological evaluation showed that the presence of a seroma cavity in combination with obstruction of the membrane prevented adjustment of the subcutaneous glucose concentration in response to changes in glycaemia. In the uncovered devices, on the other hand, changes in glycaemia were reflected in subcutaneous tissue fluid. Our results prove that it is possible to measure changes in the glucose concentration in subcutaneous tissue fluid collected with a percutaneous device. Therefore, we conclude that a percutaneous device has an application as model to study the in vivo performance of implantable glucose sensors. The use of porous membranes in such devices has to be avoided. Kluwer Academic Publishers, 2000 Gerritsen M. Department of Biomaterials, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands aut Lutterman J. Department of Internal Medicine, University Hospital St. Radboud, Nijmegen, The Netherlands aut Jansen J. Department of Biomaterials, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/8(2000-08-01), 499-503 0957-4530 11:8<499 2000 11 10856 https://doi.org/10.1023/A:1008970108339 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008970108339 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gerritsen M. Department of Biomaterials, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Lutterman J. Department of Internal Medicine, University Hospital St. Radboud, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Jansen J. Department of Biomaterials, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/8(2000-08-01), 499-503 0957-4530 11:8<499 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer