caa a22 4500 47578300X CHVBK 20180406123650.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1013009006522 doi (NATIONALLICENCE)springer-10.1023/A:1013009006522 Estrogenic activity of chemicals for dental and similar use in vitro [Elektronische Daten] [Y. Hashimoto, Y. Moriguchi, H. Oshima, J. Nishikawa, T. Nishihara, M. Nakamura] The estrogenic activities of chemicals for dental and similar use were tested by a reporter gene assay (yeast two-hybrid system) and an estrogen/estrogen receptor (ER-α) competition binding assay (fluorescence polarization system). Among the 10 chemicals [bisphenol-A (BPA), bis-2-hydroxypropyl methacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), methyl methacrylate (MMA) and 2-hydroxyethyl methacrylate (HEMA), dibutyl phthalate (DBP), n-butyl benzyl phthalate (BBP), n-butyl phthalyl n-butyl glycolate (BPBG), di-2-ethylhexyl phthalate (DEHP), and di-2-ethylhexyl adipate (DOA)], which were diluted with DMSO to concentrations ranging from 5 × 10-7 to 5 × 10-3M, and 17β-estradiol (E2) as a positive control, BPA and BBP showed estrogenic activity in these two assays, while the remaining eight chemicals did not at the concentrations tested. Additional data, together with in vivo and epidemiological examinations, are required. Such investigations should also provide information on the validity of these methods for testing the estrogenic activity of chemicals. Kluwer Academic Publishers, 2000 Hashimoto Y. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut Moriguchi Y. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut Oshima H. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut Nishikawa J. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka Suita, 565-0871, Osaka, Japan aut Nishihara T. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka Suita, 565-0871, Osaka, Japan aut Nakamura M. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/8(2000-08-01), 465-468 0957-4530 11:8<465 2000 11 10856 https://doi.org/10.1023/A:1013009006522 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1013009006522 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashimoto Y. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut NATIONALLICENCE 700 1- Moriguchi Y. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut NATIONALLICENCE 700 1- Oshima H. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut NATIONALLICENCE 700 1- Nishikawa J. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka Suita, 565-0871, Osaka, Japan aut NATIONALLICENCE 700 1- Nishihara T. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka Suita, 565-0871, Osaka, Japan aut NATIONALLICENCE 700 1- Nakamura M. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata-shi, 573-1121, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/8(2000-08-01), 465-468 0957-4530 11:8<465 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer