caa a22 4500 475783085 CHVBK 20180406123650.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1008953112683 doi (NATIONALLICENCE)springer-10.1023/A:1008953112683 Adhesion of microvascular endothelial cells to metallic implant surfaces [Elektronische Daten] [Richard Smith, M. Mosesson, A. Daniels, T. Gartner] The objective of this study was to explore the molecular mechanisms of adhesion of endothelial cells (ECs) to implant grades of titanium alloy (Ti) and stainless steel (SS), compared to tissue culture polystyrene (PS). The idea is that promotion of EC adhesion to implant surfaces during the initial stages of healing may be critical in the formation of a capillary bed intimately associated with the implant surface. Ultimately this could be expected in turn to promote bone formation close to the surface and a more stable implant/bone interface. Surfaces were coated with either peak 1 fibrinogen γAγA, fibrinogen Fr I-9, fibrinogen fragment D1, fibronectin, vitronectin, or fetal calf serum and then post-coated with bovine serum albumin (BSA) to block non-specific cell adhesion. Surfaces with BSA alone and no other protein coating were also evaluated. Fibronectin coating maximized cell adhesion on all three surfaces, and adhesion was highest on PS. BSA blocked cell adhesion to PS (and most adhesion to SS) much better than to Ti. These results provide evidence that BSA adsorption on the metal surface is unable to effectively block the adhesion of the cells to the Ti. These data may provide a basis for understanding in vivo observations that soft tissue becomes attached to a Ti surface more rapidly and with more bone formation than to SS. Evidence is also presented that αvβ3 plays an important role in adhesion of ECs to the Ti surface. These experiments also provide preliminary data which may reflect some of the features of initial EC adhesion to metal implants. ©2000 Kluwer Academic Publishers Kluwer Academic Publishers, 2000 Smith Richard Department of Orthopaedic Surgery, University of Tennessee-Campbell Clinic, 38163, Memphis, TN, USA aut Mosesson M. Milwaukee Clinical Campus, Department of Medicine, Sinai Samaritan Medical Center, University of Wisconsin Medical School, 53233, Milwaukee, Wisconsin, USA aut Daniels A. Department of Orthopaedic Surgery, University of Tennessee-Campbell Clinic, 38163, Memphis, TN, USA aut Gartner T. Department of Biology, University of Memphis, 38152, Memphis, TN, USA aut Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/5(2000-05-01), 279-285 0957-4530 11:5<279 2000 11 10856 https://doi.org/10.1023/A:1008953112683 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008953112683 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Richard Department of Orthopaedic Surgery, University of Tennessee-Campbell Clinic, 38163, Memphis, TN, USA aut NATIONALLICENCE 700 1- Mosesson M. Milwaukee Clinical Campus, Department of Medicine, Sinai Samaritan Medical Center, University of Wisconsin Medical School, 53233, Milwaukee, Wisconsin, USA aut NATIONALLICENCE 700 1- Daniels A. Department of Orthopaedic Surgery, University of Tennessee-Campbell Clinic, 38163, Memphis, TN, USA aut NATIONALLICENCE 700 1- Gartner T. Department of Biology, University of Memphis, 38152, Memphis, TN, USA aut NATIONALLICENCE 773 0- Journal of Materials Science: Materials in Medicine Kluwer Academic Publishers 11/5(2000-05-01), 279-285 0957-4530 11:5<279 2000 11 10856 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer