caa a22 4500 475783379 CHVBK 20180406123651.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1008129119065 doi (NATIONALLICENCE)springer-10.1023/A:1008129119065 Induction and characterization of pigmentation mutants in Porphyra yezoensis (Bangiales, Rhodophyta) [Elektronische Daten] [Xing-Hong Yan, Yuji Fujita, Yusho Aruga] Porphyra yezoensis Ueda conchospore germlings (1-4-cell stages) were treated with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) for inducing mutations. Three kinds of color-mutated gametophytic blades, which were composed of the mutated cells wholly, sectorially or spottedly, were obtained; and most of them were sectorially variegated blades. The highest frequency of these mutated blades was 1.3%. Four different pigmentation mutant strains were obtained by regenerating single cells and protoplasts that were enzymatically isolated from the mutated sectors of the sectorially variegated blades. The mutants were relatively stable in color in both gametophytic blade and conchocelis phases. In the two phases, each mutant strain showed characteristic differences in the in vivo absorption spectra, and had different pigment contents of major photosynthetic pigments (chlorophyll a, phycoerythrin and phycocyanin) as compared with the wild-type and with each other. The gametophytic blades from the four mutant lines showed significant differences in growth and photosynthetic rates, when they were cultured in the same conditions. By crossing the mutant with the wild-type, it was found that the color phenotypes of two mutants reported above, were resulted from two mutations in different genes, respectively. Kluwer Academic Publishers, 2000 gametophytic blade nationallicence heterozygous conchocelis nationallicence in vivo absorption spectrum nationallicence mutagen nationallicence phycobilin nationallicence pigmentation mutant nationallicence Porphyra yezoensis nationallicence Yan Xing-Hong Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-Machi, 852-8521, Nagasaki, Japan aut Fujita Yuji Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-Machi, 852-8521, Nagasaki, Japan aut Aruga Yusho Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, 156-8502, Tokyo, Japan aut Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 69-81 0921-8971 12:1<69 2000 12 10811 https://doi.org/10.1023/A:1008129119065 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008129119065 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Xing-Hong Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-Machi, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 700 1- Fujita Yuji Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-Machi, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 700 1- Aruga Yusho Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, 156-8502, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 69-81 0921-8971 12:1<69 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer