caa a22 4500 475783409 CHVBK 20180406123651.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1008178309493 doi (NATIONALLICENCE)springer-10.1023/A:1008178309493 An assessment of the AFLP method for investigating population structure in the red alga Chondrus crispus Stackhouse (Gigartinales, Florideophyceae) [Elektronische Daten] [Sylva Donaldson, Thierry Chopin, Gary Saunders] The appropriateness of the Amplified Fragment Length Polymorphism (AFLP) technique for investigating Chondrus crispus Stackhouse populations in the Maritime Provinces of Canada was assessed. The AFLP procedure was first subjected to reproducibility testing and three shortcomings were noted: 1) failure to reproduce band intensity between replicate runs for the same individual and primer pair; 2) failure of some bands to replicate; 3) lack of reproducibility for complete replicate runs for some individuals and primer pairs. In the last-mentioned case, the lack of reproducibility resulted in characteristic electropherograms indicative of weak reactions. These weak runs can be attributed to poor restriction digest/ligation reactions and/or substandard PCR, these failures ultimately resulting from low and inconsistent DNA quality. We recommend that reproducibility testing should be completed routinely in studies using the AFLP technique. In the current work, only fragments and individuals that gave reproducible results were used in subsequent analyses. The AFLP method resulted in highly variable markers within and between the populations of C. crispus included in this investigation, which prevented successful resolution of population structure. This situation could result from a lack of suitability for AFLP markers in population genetic studies, and/or too extensive genetic variation for C. crispus populations to be discerned by the AFLP technique. These two possible explanations are discussed. Kluwer Academic Publishers, 2000 AFLP nationallicence Chondrus nationallicence Florideophyceae nationallicence genetic markers nationallicence population nationallicence Rhodophyta nationallicence Donaldson Sylva Department of Biology, University of New Brunswick, E3B 5A3, Fredericton, NB, Canada aut Chopin Thierry Centre for Coastal Studies and Aquaculture, University of New Brunswick, E2L 4L5, Saint John, NB, Canada aut Saunders Gary Department of Biology, University of New Brunswick, E3B 5A3, Fredericton, NB, Canada aut Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 25-35 0921-8971 12:1<25 2000 12 10811 https://doi.org/10.1023/A:1008178309493 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008178309493 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donaldson Sylva Department of Biology, University of New Brunswick, E3B 5A3, Fredericton, NB, Canada aut NATIONALLICENCE 700 1- Chopin Thierry Centre for Coastal Studies and Aquaculture, University of New Brunswick, E2L 4L5, Saint John, NB, Canada aut NATIONALLICENCE 700 1- Saunders Gary Department of Biology, University of New Brunswick, E3B 5A3, Fredericton, NB, Canada aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 25-35 0921-8971 12:1<25 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer