caa a22 4500 475783425 CHVBK 20180406123651.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1008174115350 doi (NATIONALLICENCE)springer-10.1023/A:1008174115350 An anticoagulant proteoglycan from the marine green alga, Codium pugniformis [Elektronische Daten] [Kiminori Matsubara, Yasushi Matsuura, Kanji Hori, Keisuke Miyazawa] An anticoagulant isolated from the marine green alga Codium pugniformis was composed mainly of glucose with minor amounts of arabinose and galactose. It was highly sulfated (326 μg mg-1 polysaccharide) and contained protein(52 μg mg-1 polysaccharide) and was thus a proteoglycan. The anticoagulant properties of the purified proteoglycan were compared with those of heparin by studying the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time(TT) using normal human plasma. The proteoglycan showed similar activities to heparin, but was weaker than heparin. On the other hand, the proteoglycan did not affect PT even at the concentration at which APTT and TT were prolonged. The anticoagulation mechanism of this proteoglycan was due to the direct inhibition of thrombin and the potentiation of antithrombin III. Kluwer Academic Publishers, 2000 anticoagulant nationallicence antithrombin nationallicence Codium pugniformis nationallicence proteoglycan nationallicence sulfated polysaccharide nationallicence Matsubara Kiminori Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, 719-1197, Okayama, Japan aut Matsuura Yasushi Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, 719-1197, Okayama, Japan aut Hori Kanji Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Hiroshima, Japan aut Miyazawa Keisuke Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Hiroshima, Japan aut Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 9-14 0921-8971 12:1<9 2000 12 10811 https://doi.org/10.1023/A:1008174115350 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008174115350 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsubara Kiminori Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, 719-1197, Okayama, Japan aut NATIONALLICENCE 700 1- Matsuura Yasushi Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, 719-1197, Okayama, Japan aut NATIONALLICENCE 700 1- Hori Kanji Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Hiroshima, Japan aut NATIONALLICENCE 700 1- Miyazawa Keisuke Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 9-14 0921-8971 12:1<9 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer