caa a22 4500 475783441 CHVBK 20180406123651.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1008118917247 doi (NATIONALLICENCE)springer-10.1023/A:1008118917247 Effect of a cationic cellulosic polymer on gametophytes of Laminaria digitata (Laminariaceae, Phaeophyta): Improvement of dispersed 'sfree-living' cultures [Elektronische Daten] [Alessandra De Martino, Bernard Rousseau, Jean Claude Duval, Hugues Noël, Lise Caron] As filament aggregation is responsible for heterogeneity of Laminariales gametophyte cultures, a project was conducted to obtain stable homogeneous 'sfree-living' cultures of Laminaria digitatagametophytes. The alga was cultivated at 15 ° Cunder low light and in the presence of a cationic cellulosic polymer, JR125. With 0.1%polymer in the culture medium, the filaments were dispersed and did not adhere to the culture vessel. The absence of any effect of the closely related, but uncharged, polymer LR-250 Natrasol on filament aggregation indicated that the cationic nature of the JR125 molecule was involved in gametophyte dissociation. In the presence of JR125, the gametophytes showed active vegetative growth; the doubling time, measured as chlorophyll concentration, was 5 days. The outer surface of the cell wall was clearly modified by the polymer treatment, as observed by transmission electron microscopy, while neither the inner cell wall or cell organelles were affected. Physiological studies indicated that JR125 treatment did not disturb cell physiology, there being no effecton respiration, photosynthetic activity, sensitivity to high-light stress or modification of pigment or fluorescence characteristics. We have therefore established the conditions for maintaining a stable culture of mixed male and female Laminaria digitata gametophytes in active vegetative growth. The presence of JR 125 in the medium yields a homogeneous culture without cell physiology becoming modified. Kluwer Academic Publishers, 2000 fluorescence nationallicence homogeneous culture nationallicence JR125 polymer nationallicence Laminaria nationallicence gametophytes nationallicence photosynthesis nationallicence ultrastructural studies nationallicence De Martino Alessandra Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut Rousseau Bernard Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut Claude Duval Jean Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut Noël Hugues Daniel Jouvance Recherche & Création, Domaine Saint Hilaire Parc d'Activités de Pichaury, 13855, Aix en Provence cedex 3, France aut Caron Lise Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 83-93 0921-8971 12:1<83 2000 12 10811 https://doi.org/10.1023/A:1008118917247 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008118917247 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Martino Alessandra Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut NATIONALLICENCE 700 1- Rousseau Bernard Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut NATIONALLICENCE 700 1- Claude Duval Jean Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut NATIONALLICENCE 700 1- Noël Hugues Daniel Jouvance Recherche & Création, Domaine Saint Hilaire Parc d'Activités de Pichaury, 13855, Aix en Provence cedex 3, France aut NATIONALLICENCE 700 1- Caron Lise Laboratoire Dynamique des Membranes Végétale, U.M.R.-C.N.R.S 8543, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris cedex 05, France aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 83-93 0921-8971 12:1<83 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer