caa a22 4500 47578345X CHVBK 20180406123651.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1008137303135 doi (NATIONALLICENCE)springer-10.1023/A:1008137303135 Modification of an algal culture medium for sustained growth of a saxitoxin-producing isolate of Alexandrium minutum [Elektronische Daten] [Elizabeth Maas, Jurgen Thiele, Caryn Thompson, Rebecca Latter, Heather Brooks] In order to study saxitoxin (STX) production bymicro-algae in the laboratory, a defined algal culture medium which supports optimum growth over a longtime-period is a requirement. In the development of such a medium, a number of modifications were made to a standard algal culture medium (GP) and growth of a STX-producing isolate of Alexandrium minutum in the different formulations was assessed by measuring maximum cell densities and mean generation times (MGT). All experiments were carried out under controlled conditions in an aerobic atmosphere with increased CO2. Whilst maximum cell densities in the different modifications were similar, the MGT was significantly shortened by the addition of Tris buffer and the trace metals strontium, selenium and molybdenum. Replacement of natural with artificial seawater and removal of soil extract did not adversely affect algal growth. Five of the six media formulations supported the growth of A. minutumover a 9-month period. Kluwer Academic Publishers, 2000 Alexandrium minutum nationallicence growth medium nationallicence micro-algae nationallicence nutrients nationallicence Maas Elizabeth Department of Microbiology, University of Otago, Dunedin, New Zealand aut Thiele Jurgen Department of Microbiology, University of Otago, Dunedin, New Zealand aut Thompson Caryn Department of Mathematics and Statistics, University of Otago, Dunedin, New Zealand aut Latter Rebecca Department of Microbiology, University of Otago, Dunedin, New Zealand aut Brooks Heather Department of Microbiology, University of Otago, Dunedin, New Zealand aut Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 95-98 0921-8971 12:1<95 2000 12 10811 https://doi.org/10.1023/A:1008137303135 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008137303135 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maas Elizabeth Department of Microbiology, University of Otago, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Thiele Jurgen Department of Microbiology, University of Otago, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Thompson Caryn Department of Mathematics and Statistics, University of Otago, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Latter Rebecca Department of Microbiology, University of Otago, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Brooks Heather Department of Microbiology, University of Otago, Dunedin, New Zealand aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/1(2000-02-01), 95-98 0921-8971 12:1<95 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer