caa a22 4500 475783654 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008194627239 doi (NATIONALLICENCE)springer-10.1023/A:1008194627239 Light requirement and photosynthetic cell cultivation - Development of processes for efficient light utilization in photobioreactors [Elektronische Daten] [James Ogbonna, Hideo Tanaka] Although the potential of photosyntheticmicroorganisms for production of various metabolitesand in environmental bioremediation is recognized,their practical application has been limited by thedifficulty in supplying light efficiently tophotobioreactors. Various types of photobioreactorwith high illumination to volume ratios have beenproposed, but most are limited by cost, mass transfer,contamination, scale-up or a combination of these.The problem of light supply to photobioreactorscan be solved by developing photosynthetic cellcultivation systems where light is either substitutedor supplemented. Many strains of photosynthetic cellsare capable of heterotrophic growth under darkconditions and their heterotrophic culture can be usedfor efficient production of biomass and somemetabolites. However, light is absolutely required forefficient production of some metabolites. In suchcases, there is a need to supplement the heterotrophicwith photoautotrophic metabolism. Inphotoheterotrophic (mixotrophic) culture, thephotoautotrophic and heterotrophic metabolisms can beexploited for efficient production of usefulmetabolites but it has many problems such as processoptimization in terms of making a balance between thephotoautotrophic and heterotrophic metabolism. Another promising system is the sequentialheterotrophic/ photoautotrophic cultivation system,where the cells are cultivated heterotrophically tohigh concentrations and then passed through aphotobioreactor for accumulation of the desiredmetabolite(s). Furthermore, cyclicphotoautotrophic/heterotrophic cultivation system canbe used to achieve continuous cell growth underday/night cycles. This involves cultivating thecells photoautotrophically using solar light duringthe day and then adding controlled amount of organiccarbon source during the night for heterotrophicgrowth. In this review, these various systems arediscussed with some specific examples. Kluwer Academic Publishers, 2000 photosynthetic cells nationallicence photobioreactors nationallicence photoautotrophic culture nationallicence photoheterotrophic culture nationallicence heterotrophic culture nationallicence two step culture nationallicence cyclic culture nationallicence Ogbonna James Institute of Applied Biochemistry, University of Tsukuba, 305-8572, Tsukuba City, Japan aut Tanaka Hideo Institute of Applied Biochemistry, University of Tsukuba, 305-8572, Tsukuba City, Japan aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 207-218 0921-8971 12:3-5<207 2000 12 10811 https://doi.org/10.1023/A:1008194627239 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008194627239 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ogbonna James Institute of Applied Biochemistry, University of Tsukuba, 305-8572, Tsukuba City, Japan aut NATIONALLICENCE 700 1- Tanaka Hideo Institute of Applied Biochemistry, University of Tsukuba, 305-8572, Tsukuba City, Japan aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 207-218 0921-8971 12:3-5<207 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer