caa a22 4500 475783719 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008175217194 doi (NATIONALLICENCE)springer-10.1023/A:1008175217194 Antioxidant activities of phycocyanobilin prepared from Spirulina platensis [Elektronische Daten] [Takashi Hirata, Mikiya Tanaka, Masaki Ooike, Teppei Tsunomura, Morihiko Sakaguchi] The antioxidative activity of phycocyanobilin fromSpirulina platensis was evaluated againstoxidation of methyl linoleate in a hydrophobic systemor with phosphatidylcholine liposomes. Phycocyanobilin as well as phytochemicals includingα-tocopherol, caffeic acid and zeaxanthin,effectively inhibited the peroxidation of methyllinoleate and produced a prolonged induction period.Oxidation of phosphatidylcholine liposomes was alsocontrolled markedly by adding phycocyanobilin orα-tocopherol. Phycocyanobilin was distributedoutside in the liposomes to scavenge radicals fromAAPH and to prevent initiation of radical chainreactions. When the concentrations of phycocyanin andphycocyanobilin in the reaction mixture were adjustedequally on a phycocyanobilin basis, the activity ofphycocyanobilin was almost the same as that ofphycocyanin in the AAPH-containing reaction mixture.The antioxidizing action of phycocyanin prepared fromspray-dried Spirulina almost agreed with thatfrom fresh Spirulina in the AAPH-containingreaction mixture. These results suggest thatphycocyanobilin is responsible for the majority of theantioxidative activity of phycocyanin and may act asan effective antioxidant in a living human body. Kluwer Academic Publishers, 2000 active oxygen nationallicence blue-green alga nationallicence cyanobacteria nationallicence liposome nationallicence oxidation nationallicence phycobilin nationallicence phycobiliprotein nationallicence phycocyanin nationallicence radical nationallicence Hirata Takashi Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Tanaka Mikiya Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Ooike Masaki Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Tsunomura Teppei Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Sakaguchi Morihiko Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 435-439 0921-8971 12:3-5<435 2000 12 10811 https://doi.org/10.1023/A:1008175217194 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008175217194 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirata Takashi Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Tanaka Mikiya Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Ooike Masaki Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Tsunomura Teppei Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Sakaguchi Morihiko Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 435-439 0921-8971 12:3-5<435 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer