caa a22 4500 475783735 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008177002226 doi (NATIONALLICENCE)springer-10.1023/A:1008177002226 Extracellular polysaccharide production in outdoor mass cultures of Porphyridium sp. in flat plate glass reactors [Elektronische Daten] [Surendra Singh, Shoshana Arad, Amos Richmond] This work concerns an attempt to develop large scalecultivation of Porphyridium sp. outdoors. Theimpact on cell growth and production of solublesulphated polysaccharides of light-path length (LP)was studied in flat plate glass reactors outdoors. TheLP of the plate reactors ranged from 1.3-30 cm,corresponding to culture volumes of 3-72 L. The sidewalls of all reactors were covered, ensuring similarilluminated surfaces for all reactors. Maximal daytemperature was maintained at 26 ±1 °C.Growth conditions of pH (7.5), stirring (withcompressed air) and mineral nutrients, were optimal.Maximal volumetric concentration of the soluble sulfated polysaccharide (1.32 g L-1) was obtained in winter with the smallest light-pathreactor (1.3 cm ) at a cell density of 1.37 ×1011cells L-1. Under these conditions, theviscosity of the culture medium was also highest,being inversely proportional to the culture'slight-path. Highest areal concentration of solublepolysaccharides (60 g m-2) and areal cell density(3.01 × 1012m-2) was recorded in the 20 cmLP reactor, progressively lower values being obtainedas the light path became shorter. A similar patternwas obtained for the areal productivity ofpolysaccharides, the highest being 4.15 g m-2day-1 (considering the total illuminated reactorsurface), produced in the 20-cm LP reactor.The main sugar composition (i.e. xylose, galactose andglucose) of the sulfated polysaccharides was similarin all reactors. As viscosity increased with timeduring culture growth, there was a substantial declinein bacterial population. Cultivation throughout mostof the year provided good evidence that a light pathlength of 20 cm in flat plate reactors under theseconditions is optimal for maximal areal solublepolysaccharide production of Porphyridium sp. Kluwer Academic Publishers, 2000 areal productivity nationallicence cell mass nationallicence flat plate glass reactor nationallicence light path nationallicence polysaccharides nationallicence Porphyridium nationallicence red microalgae nationallicence Rhodophyta nationallicence viscosity nationallicence volumetric productivity nationallicence Singh Surendra Microalgal Biotechnology Lab., The Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede-Boker Campus, 84990, Israel aut Arad Shoshana Institute for Applied Biosciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel aut Richmond Amos Microalgal Biotechnology Lab., The Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede-Boker Campus, 84990, Israel aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 269-275 0921-8971 12:3-5<269 2000 12 10811 https://doi.org/10.1023/A:1008177002226 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008177002226 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Surendra Microalgal Biotechnology Lab., The Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede-Boker Campus, 84990, Israel aut NATIONALLICENCE 700 1- Arad Shoshana Institute for Applied Biosciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel aut NATIONALLICENCE 700 1- Richmond Amos Microalgal Biotechnology Lab., The Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede-Boker Campus, 84990, Israel aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 269-275 0921-8971 12:3-5<269 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer