caa a22 4500 475783794 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008195312218 doi (NATIONALLICENCE)springer-10.1023/A:1008195312218 Sorption of metals by extracellular polymers from the cyanobacterium Microcystis aeruginosa fo. flos-aquae strain C3-40 [Elektronische Daten] [Dorothy Parker, Jennifer Mihalick, John Plude, Michelle Plude, Thomas Clark, Lynn Egan, Joshua Flom, L.C. Rai, H.D. Kumar] The sorption of cadmium (II), copper (II), lead (II),manganese (II), and zinc (II) by purified capsularpolysaccharide from the cyanobacterium Microcystis aeruginosafo. flos-aquae strainC3-40 was examined by four methods: equilibriumdialysis, metal removal from solution as detected byvoltammetry, metal accumulation by capsule-containingalginate beads, and calorimetry. The polysaccharide'ssaturation binding capacities for these metals rangedfrom 1.2 to 4 mmol of metal g-1 of capsule, whichcorresponds to 1 metal equivalent per 2 to 4saccharide subunits of the polymer. Competitionbetween paired metals was tested with simultaneous andsequential additions of metal. Cadmium (II) andlead (II), as well as lead (II) and zinc (II), competedrelatively equally and reciprocally for polymerbinding sites. In contrast, manganese (II) stronglyinhibited the binding of cadmium (II) and lead (II), butitself was not substantially inhibited by either theprior or simultaneous adsorption of cadmium (II) or lead (II).The data are interpreted with respect to overlap ofbinding sites and possibilities of altered polymerconformation or solvation. Calorimetric studies oflead (II) and cadmium (II) association reactions withthe polysaccharide suggest that the enthalpies aresmall and that the reactions may be driven by entropy. Kluwer Academic Publishers, 2000 cyanobacterium nationallicence Cyanophyta nationallicence Microcystis nationallicence capsule nationallicence slime layer nationallicence metal nationallicence adsorption nationallicence polysaccharide nationallicence equilibrium dialysis nationallicence voltammetry nationallicence Parker Dorothy Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A. aut Mihalick Jennifer Department of Chemistry, University of Wisconsin Oshkosh, 54901, Oshkosh, WI, U.S.A. aut Plude John Department of Chemistry, University of Wisconsin Oshkosh, 54901, Oshkosh, WI, U.S.A. aut Plude Michelle Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A. aut Clark Thomas Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A. aut Egan Lynn Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A. aut Flom Joshua Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A. aut Rai L.C. Department of Botany, Banaras Hindu University, 221005, Varanasi, UP, India aut Kumar H.D. Department of Botany, Banaras Hindu University, 221005, Varanasi, UP, India aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 219-224 0921-8971 12:3-5<219 2000 12 10811 https://doi.org/10.1023/A:1008195312218 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008195312218 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Parker Dorothy Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Mihalick Jennifer Department of Chemistry, University of Wisconsin Oshkosh, 54901, Oshkosh, WI, U.S.A aut NATIONALLICENCE 700 1- Plude John Department of Chemistry, University of Wisconsin Oshkosh, 54901, Oshkosh, WI, U.S.A aut NATIONALLICENCE 700 1- Plude Michelle Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Clark Thomas Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Egan Lynn Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Flom Joshua Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 92037, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Rai L.C. Department of Botany, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 700 1- Kumar H.D. Department of Botany, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 219-224 0921-8971 12:3-5<219 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer