caa a22 4500 475783808 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008123000002 doi (NATIONALLICENCE)springer-10.1023/A:1008123000002 Mixotrophic growth of Phaeodactylum tricornutum on glycerol: growth rate and fatty acid profile [Elektronische Daten] [M.C. Cerón Garcí, J.M. Fernández Sevilla, F.G. Acién Fernández, E. Molina Grima, F. García Camacho] Mixotrophic growth of the eicosapentaenoic acid (EPA)producing diatom Phaeodactylum tricornutum UTEX640 was carried out in 1-L batch cultures under anexternal irradiance of 165 μmol photons m-2s-1 by supplementing the inorganic culture mediumwith glycerol. The effect on the growth and the fattyacid profile was studied for different initialglycerol concentrations (0-0.1 M). The optimalglycerol concentration was 0.1 M.A lag phase was observed at high glycerolconcentrations. The present study also shows thatsuccessive additions of glycerol at 0.1M concentrationand using ammonium chloride as a nitrogen sourceremarkably increased the maximum biomass concentration(16.2 g L-1) and maximum biomass productivity(61.5 mg L-1 h-1). These values wererespectively 9 and 8-fold higher than in thephotoautotrophically grown control. The level ofsaponifiable lipids in mixotrophically cultured cellswas significantly higher than in photoautotrophicallycultured cells and increased with the glycerolconcentration in the medium. The concentration ofstorage lipids, saturated and monounsaturated fattyacids, were enhanced but the EPA content did notchange significantly. The EPA content was around 2.2%of biomass dry weight. The maximum EPA yield was33.5 mg L-1 d-1 and was obtained in aculture containing 0.1 M glycerol, supplementedperiodically by ammonium chloride. This productivitywas 10-fold higher than the EPA productivity obtainedunder mixotrophic conditions. Kluwer Academic Publishers, 2000 eicosapentaenoic acid nationallicence microalga nationallicence mixotrophic growth nationallicence Phaeodactylum tricornutum nationallicence Cerón Garcí M.C. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut Fernández Sevilla J.M. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut Acién Fernández F.G. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut Molina Grima E. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut García Camacho F. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 239-248 0921-8971 12:3-5<239 2000 12 10811 https://doi.org/10.1023/A:1008123000002 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008123000002 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cerón Garcí M.C. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut NATIONALLICENCE 700 1- Fernández Sevilla J.M. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut NATIONALLICENCE 700 1- Acién Fernández F.G. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut NATIONALLICENCE 700 1- Molina Grima E. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut NATIONALLICENCE 700 1- García Camacho F. Departamento de Ingeniería Química, Universidad de Almería, E-04071, Almería, Spain aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 239-248 0921-8971 12:3-5<239 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer