caa a22 4500 475783867 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008107229005 doi (NATIONALLICENCE)springer-10.1023/A:1008107229005 Cryopreservation-recalcitrance in microalgae: novel approaches to identify and avoid cryo-injury [Elektronische Daten] [John Day, Roland Fleck, Erica Benson] Standard two-step freezing protocols areunsatisfactory for Euglena gracilis and manyother microalgae, particularly those with larger cellsizes, complex morphologies and/or those susceptibleto environmental stress. Using techniques that allowmechanisms of injury and sites of damage to beidentified (e.g. monitoring oxygen evolving capacity,detection of •OH, microscopic visualisation ofintracellular ice and structural/ultrastructuraldamage), it is possible to improve conventionalcryopreservation methodologies. In E. gracilisthis has resulted in the development of protocolswhich increased post-thaw viability levels from 0 to20%. Alternative cryoprotection strategies testedincluded vitrification and encapsulation/dehydration.Vitrification was unsuccessful due to the hightoxicity of the solutions. Encapsulation/dehydration,with or without two-step cooling were suitable forcryopreservation of E. gracilis, the latterresulted in the highest levels of post-thaw viability(40%) and viability was maintained after 12 monthsstorage. Kluwer Academic Publishers, 2000 algae nationallicence cryo-injury nationallicence cryopreservation nationallicence culture collection nationallicence cryo-storage nationallicence Euglenagracilis nationallicence encapsulation/dehydration nationallicence vitrification nationallicence Day John Culture Collection of Algae and Protozoa, Centre for Ecology & Hydrology Windermere, Far Sawrey, NERC, Ambleside, Cumbria, LA22, 0LP, UK aut Fleck Roland Department of Soil, Crop and Atmospheric Sciences, Cornell University, 14853, Ithaca, NY, USA aut Benson Erica Plant Conservation Biotechnology Group, Division of Molecular and Life Sciences, School of Science and Engineering, University of Abertay Dundee, Bell Street, DD1 1HG, Dundee, Scotland, UK aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 369-377 0921-8971 12:3-5<369 2000 12 10811 https://doi.org/10.1023/A:1008107229005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008107229005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Day John Culture Collection of Algae and Protozoa, Centre for Ecology & Hydrology Windermere, Far Sawrey, NERC, Ambleside, Cumbria, LA22, 0LP, UK aut NATIONALLICENCE 700 1- Fleck Roland Department of Soil, Crop and Atmospheric Sciences, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Benson Erica Plant Conservation Biotechnology Group, Division of Molecular and Life Sciences, School of Science and Engineering, University of Abertay Dundee, Bell Street, DD1 1HG, Dundee, Scotland, UK aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 369-377 0921-8971 12:3-5<369 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer