caa a22 4500 475783913 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008114929490 doi (NATIONALLICENCE)springer-10.1023/A:1008114929490 Nitrogen-fixing cyanobacteria as gene delivery system for expressing mosquitocidal toxins of Bacillus thuringiensis ssp. israelensis [Elektronische Daten] [S. Boussiba, X-Q. Wu, E. Ben-Dov, A. Zarka, A. Zaritsky] Classical biological control is the most successfuland promising way to replace chemical pesticides. Thesubspecies israelensis of Bacillusthuringiensis (Bti) is a safe and efficient agent tocontrol mosquito larvae and hence mosquito-bornediseases. One approach to overcome the low efficacyand short half-life in nature of current formulationsof Bti is by expressing the toxin genes in recombinantcyanobacteria as a delivery system. Attempts toexpress Bti toxin in cyanoabcteria have been carriedout during the last ten years. Toxicities of thetransgenic strains were however very low, even underregulation of strong promoters, too low to beeffective in vivo. Two Bti Cry proteins haverecently been co-expressed in the filamentousnitrogen-fixing cyanobacterium Anabaena PCC7120, resulting in clones with the highest toxicitiesand stabilities ever reached so far. However, toobtain a long-lasting preparation, it would be usefulto express Bti toxin genes in cyanobacterial strainsisolated from nature. This approach requiresdevelopment of a system for effective transformationinto such strains. Releasing such recombinant strainsto open environments is still a major obstacle inexploiting this biotechnology. Kluwer Academic Publishers, 2000 Anabaena PCC 7120 nationallicence Bacillusthuringiensis ssp. israelensis nationallicence biologicalcontrol nationallicence cyanobacteria nationallicence gene cloning nationallicence Boussiba S. Microalgal Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, Blaustein Institute for Desert Research, Ben-Gurion University, 84990, Sede-Boker, Israel aut Wu X-Q Microalgal Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, Blaustein Institute for Desert Research, Ben-Gurion University, 84990, Sede-Boker, Israel aut Ben-Dov E. Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 83105, Be'er-Sheva, Israel aut Zarka A. Microalgal Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, Blaustein Institute for Desert Research, Ben-Gurion University, 84990, Sede-Boker, Israel aut Zaritsky A. Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 83105, Be'er-Sheva, Israel aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 461-467 0921-8971 12:3-5<461 2000 12 10811 https://doi.org/10.1023/A:1008114929490 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008114929490 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boussiba S. Microalgal Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, Blaustein Institute for Desert Research, Ben-Gurion University, 84990, Sede-Boker, Israel aut NATIONALLICENCE 700 1- Wu X-Q Microalgal Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, Blaustein Institute for Desert Research, Ben-Gurion University, 84990, Sede-Boker, Israel aut NATIONALLICENCE 700 1- Ben-Dov E. Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 83105, Be'er-Sheva, Israel aut NATIONALLICENCE 700 1- Zarka A. Microalgal Biotechnology Laboratory, Albert Katz Department of Dryland Biotechnologies, Blaustein Institute for Desert Research, Ben-Gurion University, 84990, Sede-Boker, Israel aut NATIONALLICENCE 700 1- Zaritsky A. Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 83105, Be'er-Sheva, Israel aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 461-467 0921-8971 12:3-5<461 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer