caa a22 4500 475783972 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008151526680 doi (NATIONALLICENCE)springer-10.1023/A:1008151526680 Scale-up aspects of photobioreactors: effects of mixing-induced light/dark cycles [Elektronische Daten] [Marcel Janssen, Lucas de Bresser, Teun Baijens, Johannes Tramper, Luuc Mur, Jan Snel, René Wijffels] The green micro-algae Chlamydomonas reinhardtiiand Dunaliella tertiolecta were cultivated undermedium-duration square-wave light/dark cycles with acycle time of 15 s. These cycles were used to simulatethe light regime experienced by micro-algae inexternally-illuminated (sunlight) air-lift loopbioreactors with internal draft tube. Biomass yieldin relation to light energy was determined as gprotein per mol of photons (400-700 nm). Between 600and 1200 μmol m-2 s-1 the yield at a10/5 s light/dark cycle was equal to the yield atcontinuous illumination. Consequently, provided thatthe liquid circulation time is 15 s, a considerabledark zone seems to be allowed in the interior ofair-lift loop photobioreactors (33% v/v) without lossof light utilization efficiency. However, at a 5/10 slight/dark cycle, corresponding to a 67% v/v darkzone, biomass yield decreased. Furthermore, bothalgae, C. reinhardtii and D. tertiolecta,responded similarly to these cycles with respect tobiomass yield. This was interesting because they werereported to exhibit a different photoacclimationstrategy. Finally, it was demonstrated that D.tertiolecta was much more efficient at low (average)photon flux densities (57-370 μmol m-2s-1) than at high PFDs (> 600 μmol m-2s-1) and it was shown that D. tertiolectawas cultivated at a sub-optimal temperature (20 °C). Kluwer Academic Publishers, 2000 microalgae nationallicence Chlamydomonas reinhardtii nationallicence Dunaliellatertiolecta nationallicence light/dark cycles nationallicence photobioreactors nationallicence light utilizationefficiency nationallicence biomass yield nationallicence quantum yield nationallicence Janssen Marcel Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut de Bresser Lucas Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut Baijens Teun Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut Tramper Johannes Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut Mur Luuc Amsterdam Research Institute for Substances in Ecosystems, University of Amsterdam, The Netherlands aut Snel Jan Laboratory for Plant Physiology, Wageningen University, The Netherlands aut Wijffels René Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 225-237 0921-8971 12:3-5<225 2000 12 10811 https://doi.org/10.1023/A:1008151526680 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008151526680 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Janssen Marcel Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- de Bresser Lucas Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Baijens Teun Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Tramper Johannes Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Mur Luuc Amsterdam Research Institute for Substances in Ecosystems, University of Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Snel Jan Laboratory for Plant Physiology, Wageningen University, The Netherlands aut NATIONALLICENCE 700 1- Wijffels René Food and Bioprocess Engineering Group, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 225-237 0921-8971 12:3-5<225 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer