caa a22 4500 475784014 CHVBK 20180406123652.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008162812651 doi (NATIONALLICENCE)springer-10.1023/A:1008162812651 Increased production of extra- and intracellular metal-ligands in phytoplankton exposed to copper and cadmium [Elektronische Daten] [R. Pistocchi, M.A. Mormile, F. Guerrini, G. Isani, L. Boni] Several diatom and dinoflagellate species isolatedfrom the Adriatic Sea, grown in batch cultures in f/2medium, were exposed to two different concentrationsof Cu and Cd. The concentrations chosen caused areduction of growth, but were not lethal. Thespecies able to tolerate the highest Cu concentrations(in the order of 0.2-0.5 mg L-1) were diatoms,while some diatoms and all the dinoflagellates wereinhibited by concentrations between 0.01 and 0.05 mgCu L-1. Cadmium was less toxic in that it did notaffect diatom or dinoflagellate growth up to aconcentration of 0.2 mg L-1. Increasedproduction of extracellular polysaccharides was foundto be a general response to the presence of thepollutant. Cylindrotheca fusiformis, Achnanthesbrevipes and Prorocentrum micans wereinvestigated for their production of intracellularmetal-ligands. Molecules with Cd-binding propertiesand having a molecular weight comparable with that ofphytochelatins were found only in C.fusiformis, and their presence showed both adose- and time-dependent response. The presence orabsence of intracellular ligands in these algae isconsistent with their ability to exclude the metalextracellularly. Kluwer Academic Publishers, 2000 cadmium nationallicence copper nationallicence diatoms nationallicence dinoflagellates nationallicence extracellular polysaccharides nationallicence phytochelatins nationallicence Pistocchi R. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut Mormile M.A. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut Guerrini F. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut Isani G. Dipartimento di Biochimica, Sezione di Biochimica Veterinaria, Università di Bologna, Ozzano (BO), Italy aut Boni L. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 469-477 0921-8971 12:3-5<469 2000 12 10811 https://doi.org/10.1023/A:1008162812651 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008162812651 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pistocchi R. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut NATIONALLICENCE 700 1- Mormile M.A. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut NATIONALLICENCE 700 1- Guerrini F. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut NATIONALLICENCE 700 1- Isani G. Dipartimento di Biochimica, Sezione di Biochimica Veterinaria, Università di Bologna, Ozzano (BO), Italy aut NATIONALLICENCE 700 1- Boni L. Scienze Ambientali, Università di Bologna, Via Tombesi dall'Ova 55, 48100, Ravenna, Italy aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 469-477 0921-8971 12:3-5<469 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer