caa a22 4500 475784022 CHVBK 20180406123653.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1008149529914 doi (NATIONALLICENCE)springer-10.1023/A:1008149529914 Effect of photosynthesis on pH variation in cyanobacterial biofilms from Roman catacombs [Elektronische Daten] [P. Albertano, L. Bruno, D. D'Ottavi, D. Moscone, G. Palleschi] Cyanobacterial biofilms present on stone surfaces inRoman hypogea were studied with the aim of assessingtheir deteriogenic activity on the colonisedsubstrata. In order to achieve this, non-destructivemethods were developed and applied to measure pHvariation induced via photosynthesis and respirationin representative cyanobacteria from Roman catacombs.Amperometric and potentiometric microsensors were alsoused on Scytonema biofilms in culture in orderto measure photosynthesis and assess pH decreases andincreases during dark-light periods. Measurementsof pH showed that, starting with values slightly belowneutral, the pH in Scytonema biofilms increasedby 0.24-0.77 units in the transition from dark to1000 μmol photon m-2 s-1 irradiance.Comparison of photosynthesis and pH curves recordedsimultaneously on the same artificial biofilm showeda maximum increase in pH value at irradiances higherthan those saturating photosynthesis. Alkalinisationof the substrate during illumination occurred to asufficient extent to induce precipitation of mineralcompounds, especially on calcareous substrates such asthose present in Roman hypogea. Kluwer Academic Publishers, 2000 cyanobacterial biofilms nationallicence Scytonema nationallicence mineral biodeterioration nationallicence pH microelectrodes nationallicence damage diagnosis nationallicence Albertano P. Department of Biology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut Bruno L. Department of Biology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut D'Ottavi D. Department of Chemical Sciences and Technology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut Moscone D. Department of Chemical Sciences and Technology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut Palleschi G. Department of Chemical Sciences and Technology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 379-384 0921-8971 12:3-5<379 2000 12 10811 https://doi.org/10.1023/A:1008149529914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008149529914 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Albertano P. Department of Biology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Bruno L. Department of Biology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut NATIONALLICENCE 700 1- D'Ottavi D. Department of Chemical Sciences and Technology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Moscone D. Department of Chemical Sciences and Technology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Palleschi G. Department of Chemical Sciences and Technology, University of Rome 'sTor Vergata', via della Ricerca scientifica, 00133, Rome, Italy aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/3-5(2000-10-01), 379-384 0921-8971 12:3-5<379 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer