caa a22 4500 475784073 CHVBK 20180406123653.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1008176409864 doi (NATIONALLICENCE)springer-10.1023/A:1008176409864 Cloning and characterization of G+C-rich, highly repeated DNA sequences from Porphyra yezoensis (laver), Rhodophyta [Elektronische Daten] [Yuzuru Mizukami, Hitoshi Kito, Masahiko Kunimoto, Masahiro Kobayashi] G+C-rich sequences in the genomic DNA of Porphyrayezoensis (laver) were cloned and characterized. Sequence analyses of the genomic DNA inserted in fiveclones showed that the DNA contained long G+C-richstretches of more than 200 bp. These stretchesconsisted of more than 80% G+C residues. TheG+C-rich sequences were highly repeated andinterspersed throughout the genome of P.yezoensis and constituted about 6.0-6.6% of thegenome. Parts of these sequences were tandem repeatedin arrays. Hybridization experiments showed thatthese highly repeated, interspersed G+C-rich sequenceswere present in other species of Porphyra, butnot in species of the genera Grateloupia and Gelidium, suggesting that these sequences mightevolve rapidly among genomes, species and genera. Kluwer Academic Publishers, 2000 G+C-rich sequence nationallicence interspersed DNA nationallicence Porphyra yezoensis nationallicence tandem repeated DNA nationallicence Mizukami Yuzuru National Fisheries University, Shimonoseki, 759-6595, Yamaguchi, Japan aut Kito Hitoshi National Fisheries University, Shimonoseki, 759-6595, Yamaguchi, Japan aut Kunimoto Masahiko National Fisheries University, Shimonoseki, 759-6595, Yamaguchi, Japan aut Kobayashi Masahiro Ishigaki Tropical Station, Seikai National Fisheries Research Institute, Ishigaki, 907-0451, Okinawa, Japan aut Journal of Applied Phycology Kluwer Academic Publishers 12/2(2000-03-01), 131-138 0921-8971 12:2<131 2000 12 10811 https://doi.org/10.1023/A:1008176409864 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008176409864 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizukami Yuzuru National Fisheries University, Shimonoseki, 759-6595, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Kito Hitoshi National Fisheries University, Shimonoseki, 759-6595, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Kunimoto Masahiko National Fisheries University, Shimonoseki, 759-6595, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Kobayashi Masahiro Ishigaki Tropical Station, Seikai National Fisheries Research Institute, Ishigaki, 907-0451, Okinawa, Japan aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/2(2000-03-01), 131-138 0921-8971 12:2<131 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer