caa a22 4500 475784081 CHVBK 20180406123653.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1008132210772 doi (NATIONALLICENCE)springer-10.1023/A:1008132210772 Inhibitory effect of phycocyanin from Spirulina platensis on the growth of human leukemia K562 cells [Elektronische Daten] [Yufeng Liu, Lizhi Xu, Ni Cheng, Lijun Lin, Chengwu Zhang] The effect of phycocyanin from Spirulinaplatensis on the growth of human chronic myelogenousleukemia-blast crisis K562 cells was studied bysemi-solid agar assay and cell viability measurement. Phycocyanin significantly inhibited the growth of K562cells in a dose-dependent manner. The IC50 value ofthe phycocyanin was 72.5 mg L-1. After the K562cells were cultured with phycocyanin for 6 days, flowcytometric assays showed that more K562 cells wereblocked to progress through S-phase and arrested at G1phase. DNA fragmentation assay indicated that therewas no ladder of DNA fragments of approximately 200-bpmultiples, indicating that apoptosis had not occurred. Western blot analysis showed that Bcl-2 protein wasexpressed, but its level remained unchanged, whereasthe expression level of c-myc increased. Thesefindings suggest that phycocyanin may be able toinhibit the growth of K562 cells by pathways otherthan apoptosis, and that changed a expression patternof the c-myc protein may be involved in such inhibition. Kluwer Academic Publishers, 2000 Bcl-2 nationallicence C-myc nationallicence growth inhibition nationallicence G1 arrest nationallicence K562 cells nationallicence Phycocyanin nationallicence Spirulina platensis nationallicence Liu Yufeng Medical School, Nanjing University, 210093, Nanjing, PR of China aut Xu Lizhi Medical School, Nanjing University, 210093, Nanjing, PR of China aut Cheng Ni Department of Biological Science & Technology, Nanjing University, 210093, Nanjing, PR of China aut Lin Lijun Medical School, Nanjing University, 210093, Nanjing, PR of China aut Zhang Chengwu Department of Applied Biochemistry, Nanjing University of Chemical Technology, 210009, Nanjing, PR of China aut Journal of Applied Phycology Kluwer Academic Publishers 12/2(2000-03-01), 125-130 0921-8971 12:2<125 2000 12 10811 https://doi.org/10.1023/A:1008132210772 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008132210772 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yufeng Medical School, Nanjing University, 210093, Nanjing, PR of China aut NATIONALLICENCE 700 1- Xu Lizhi Medical School, Nanjing University, 210093, Nanjing, PR of China aut NATIONALLICENCE 700 1- Cheng Ni Department of Biological Science & Technology, Nanjing University, 210093, Nanjing, PR of China aut NATIONALLICENCE 700 1- Lin Lijun Medical School, Nanjing University, 210093, Nanjing, PR of China aut NATIONALLICENCE 700 1- Zhang Chengwu Department of Applied Biochemistry, Nanjing University of Chemical Technology, 210009, Nanjing, PR of China aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/2(2000-03-01), 125-130 0921-8971 12:2<125 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer