caa a22 4500 475784146 CHVBK 20180406123653.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1008119125911 doi (NATIONALLICENCE)springer-10.1023/A:1008119125911 Endogenous and exogenous elicitors of a hypersensitive response in Gracilaria conferta (Rhodophyta) [Elektronische Daten] [Florian Weinberger, Michael Friedlander] Certain forms of oligocellulose and certainbacterially excreted peptides were identified asendogenous and exogenous elicitors, respectively, ofa tip bleaching response in Gracilaria conferta(Schousboe ex Montagne) J. et G. Feldmann. Thehalf-maximal tip bleaching response was observed when31.1 μM cellobiose or 11.6 μM cellotetraosewere present in the growth medium. In contrast, noresponse was detected after exposure to glucose,cellotriose, cellopentaose or maltooligosaccharides.The response was thus strongly dependent on themolecular size of the oligocellulose and onlysaccharides that consisted of an even number ofglucose residues were elicitor-active. Three bacterialspecies that had earlier been identified as potentialinducers of the tip bleaching symptom excretedelicitor-active compounds into the growth medium.These compounds were protease-sensitive and thuspeptides or proteins. The tip bleaching-inducingcompound that was excreted by one Cytophaga-likeorganism was partially purified. It could be extractedfrom culture supernatants with chloroform and itsmolecular size was between 700 and 1500 Da,corresponding with a structure of 4-20 amino acids.Various endogenous and exogenous elicitors are thusrecognized by G. conferta and allow this alga torespond hypersensitively to the maceration of its cellwall skeleton or just to the presence of certainepiphytic organisms. Kluwer Academic Publishers, 2000 cellulose nationallicence epiphytic bacteria nationallicence Gracilaria conferta nationallicence oligosaccharide nationallicence peptide,plant-pathogen interactions nationallicence Weinberger Florian Israel Oceanographic & Limnological Research, National Institute of Oceanography, P.O.Box 8030, 31080, Haifa, Israel aut Friedlander Michael Israel Oceanographic & Limnological Research, National Institute of Oceanography, P.O.Box 8030, 31080, Haifa, Israel aut Journal of Applied Phycology Kluwer Academic Publishers 12/2(2000-03-01), 139-145 0921-8971 12:2<139 2000 12 10811 https://doi.org/10.1023/A:1008119125911 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1008119125911 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weinberger Florian Israel Oceanographic & Limnological Research, National Institute of Oceanography, P.O.Box 8030, 31080, Haifa, Israel aut NATIONALLICENCE 700 1- Friedlander Michael Israel Oceanographic & Limnological Research, National Institute of Oceanography, P.O.Box 8030, 31080, Haifa, Israel aut NATIONALLICENCE 773 0- Journal of Applied Phycology Kluwer Academic Publishers 12/2(2000-03-01), 139-145 0921-8971 12:2<139 2000 12 10811 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer