caa a22 4500 475785274 CHVBK 20180406123656.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004310050051 doi (NATIONALLICENCE)springer-10.1007/s004310050051 Elevated gene expression of interleukin-8 in cord blood is a sensitive marker for neonatal infection [Elektronische Daten] [Reinhard Berner, Barbara Tüxen, Andreas Clad, Johannes Forster, Matthias Brandis] Bacterial infection is a major cause of neonatal morbidity and mortality. Early diagnosis is essential for a successful treatment and outcome. Cytokine plasma levels are suggested to be sensitive parameters for the diagnosis of neonatal sepsis. The aim of this study was to assess cytokine mRNA expression in cord blood cells as a marker for neonatal infection. In a prospective study, cord blood samples of neonates with septic bacterial infection were analyzed qualitatively and semiquantitatively by reverse transcriptase-polymerase chain reaction (RT-PCR) for mRNA expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, as well as for IL-8 cord plasma levels. Results were compared to those of non-septic neonates. A method was used requiring only a microvolume (25 μl or less) of cord blood. Cord plasma levels of IL-8 were significantly elevated in septic infants (n = 9) when compared to infants with not confirmed sepsis (n = 22) and healthy infants that served as controls (n = 68) (median 1,686 vs 262.7 vs 33.1 pg/ml, P < 0.001). The presence of IL-6 and TNF-α gene expression was observed more frequently in septic than in non-septic patients; sensitivity, however, reached only 56 and 67%, respectively. When using a semiquantitative approach for analyzing IL-8 mRNA levels, a high sensitivity (86%) and specificity (96%) for the detection of sepsis was achieved. A new method for the early diagnosis of neonatal infection is described measuring cytokine mRNA in neonatal cord blood cells. With this molecular approach only a microvolume of blood is required for analysis. Springer-Verlag Berlin Heidelberg, 2000 Key words Neonatal sepsis nationallicence Cytokines nationallicence mRNA nationallicence Reverse transcriptase-polymerase chain reaction nationallicence Berner Reinhard University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut Tüxen Barbara University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut Clad Andreas University Hospital of Obstetrics and Gynecology, Hugstetter Strasse 1, 79106 Freiburg, Germany, DE aut Forster Johannes University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut Brandis Matthias University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut https://doi.org/10.1007/s004310050051 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004310050051 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berner Reinhard University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut NATIONALLICENCE 700 1- Tüxen Barbara University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut NATIONALLICENCE 700 1- Clad Andreas University Hospital of Obstetrics and Gynecology, Hugstetter Strasse 1, 79106 Freiburg, Germany, DE aut NATIONALLICENCE 700 1- Forster Johannes University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut NATIONALLICENCE 700 1- Brandis Matthias University Children's Hospital, Mathildenstrasse 1, 79106 Freiburg, Germany e-mail: berner@kkl200.ukl.uni-freiburg.de Tel.: +49-761-2704300; Fax: +49-761-2704481, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer