caa a22 4500 475785436 CHVBK 20180406123656.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/PL00008344 doi (NATIONALLICENCE)springer-10.1007/PL00008344 Inhibitory effect of meconium on pulmonary surfactant function tested in vitro using the stable microbubble test [Elektronische Daten] [Myung Ho Oh, Chong Woo Bae] Meconium aspiration syndrome is related to mechanical obstruction of the airways and subsequent chemical pneumonitis. It has also been suggested that meconium causes inhibition of surfactant function. To assess its inhibitory effect on surfactant function in vitro, we used a stable microbubble (SM) test that was thought to reflect the adequacy of pulmonary surfactant. The mixtures were prepared by adding serial dilutions of human meconium to various concentrations of Surfactant-TA (SurfactenTM). The SM count at each concentration of surfactant significantly increased with the increasing concentration of surfactant. This shows that the SM test closely reflects the quantified function of surfactant. When various concentrations of meconium were added to the surfactant concentration of 0.05 mg/ml and 0.25 mg/ml, the SM test results were decreased even at low concentrations of meconium. Also the increase in the meconium concentration caused a decrease in the SM test result, which was dependent on the surfactant and the meconium concentration, accordingly. These results suggest that meconium inhibits surfactant function. Conclusion The stable microbubble test is an effective indirect method that tests the changes in surfactant quantity. In the in vitro experiment, we observed an inhibitory effect of meconium on the surfactant activity using the stable microbubble test. Springer-Verlag Berlin Heidelberg, 2000 Key words Meconium nationallicence Pulmonary surfactant nationallicence Stable microbubble test nationallicence Surfactant-meconium mixtures nationallicence Respiratory distress syndrome nationallicence Oh Myung Ho Department of Paediatrics, KonKuk University Hospital, 620-5 Kyohyun-dong, ChungJu, Chungbuk 380-060, Korea e-mail: omhp@kku.edu Tel.: +82-441-8452501 ext. 222; Fax: +82-441-8444826, KR aut Bae Chong Woo Department of Paediatrics, Kyunghee University Hospital, Seoul 130-702, Korea, KR aut https://doi.org/10.1007/PL00008344 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008344 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oh Myung Ho Department of Paediatrics, KonKuk University Hospital, 620-5 Kyohyun-dong, ChungJu, Chungbuk 380-060, Korea e-mail: omhp@kku.edu Tel.: +82-441-8452501 ext. 222; Fax: +82-441-8444826, KR aut NATIONALLICENCE 700 1- Bae Chong Woo Department of Paediatrics, Kyunghee University Hospital, Seoul 130-702, Korea, KR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer