caa a22 4500 475786858 CHVBK 20180406123700.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004310051286 doi (NATIONALLICENCE)springer-10.1007/s004310051286 Polymerase chain reaction detection of Bartonella henselae bacteraemia in an immunocompetent child with cat-scratch disease [Elektronische Daten] [Raffaele Del Prete, Donato Fumarola, Sandro Ungari, Luciana Fumarola, Giuseppe Miragliotta] A case of Bartonella henselae bacteraemia is reported in an immunocompetent 8-year-old boy with cat-scratch disease. Serology to B. henselae, diagnosed by polymerase chain reaction, was positive. DNA was extracted from peripheral whole blood and amplified with specific primers targeting the htrA gene of B. henselae. A non-isotopic hybridization assay with a species-specific oligonucleotide probe was used to detect the amplified product. Conclusion The polymerase chain reaction can be used for the rapid laboratory diagnosis of bacteraemia in cat-scratch disease. Springer-Verlag Berlin Heidelberg, 2000 Key wordsBartonella henselae nationallicence Polymerase chain reaction nationallicence Cat-scratch disease nationallicence Bacteraemia nationallicence Del Prete Raffaele Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut Fumarola Donato Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut Ungari Sandro I and III Division, IRCCS Paediatric Hospital, "Bambino Gesù”, Rome, Italy, IT aut Fumarola Luciana Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut Miragliotta Giuseppe Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut https://doi.org/10.1007/s004310051286 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004310051286 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Del Prete Raffaele Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut NATIONALLICENCE 700 1- Fumarola Donato Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut NATIONALLICENCE 700 1- Ungari Sandro I and III Division, IRCCS Paediatric Hospital, "Bambino Gesù”, Rome, Italy, IT aut NATIONALLICENCE 700 1- Fumarola Luciana Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut NATIONALLICENCE 700 1- Miragliotta Giuseppe Section of Microbiology and Immunology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Piazza G. Cesare 4, 70124 Bari, Italy e-mail: rdelprete@midim.uniba.it Tel.: +33-80-5478486; Fax: +33-80-5478537, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer