caa a22 4500 475787196 CHVBK 20180406123701.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s004310000581 doi (NATIONALLICENCE)springer-10.1007/s004310000581 Cell death and lung cell histology in meconium aspirated newborn rabbit lung [Elektronische Daten] [Alexander Zagariya, Rama Bhat, Bruce Uhal, Shankararao Navale, Michael Freidine, Dharmapuri Vidyasagar] Meconium aspiration syndrome (MAS) is a major cause of newborn mortality and morbidity. In this study we investigated the inflammatory responses and morphological changes in the newborn lung to debris-free meconium instillation. We developed a model for studies of MAS using 2-week-old rabbit pups. Cell death was assessed by DNA staining and detection of DNA fragmentation by in situ end labeling. Cell death was seen in association with an increase of inflammatory cytokines levels, studied by ELISA. Necrotic cells were detected by staining of lavage cells with ethidium bromide and 4′,6′-diamino-2′-phenylidon. Meconium instillation resulted selectively in loss of airway and alveolar epithelial cells followed by cell death, which increased with time. Necrotic cells looked smaller and damaged with maximal counts at 24 h after instillation. Conclusion Meconium instillation into lungs caused massive cell death, possibly by apoptosis, and necrosis that may have been activated by the inflammatory cytokine production. Springer-Verlag Berlin Heidelberg, 2000 Key words Cell death nationallicence Cytokines nationallicence In situ DNA end labeling nationallicence Lungs nationallicence Meconium nationallicence AbbreviationsDAPI 4′,6′-diamino-2′-phenylidon nationallicence IL interleukin nationallicence ISEL in situ DNA end labeling nationallicence MAS meconium aspiration syndrome nationallicence PBS phosphate buffer solution nationallicence PGE2 prostaglandin E2 nationallicence TNFα tumor necrosis factor α nationallicence Zagariya Alexander Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut Bhat Rama Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut Uhal Bruce Cardiovascular Institute, Michael Reese Hospital, Chicago, IL 60616, USA, US aut Navale Shankararao Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut Freidine Michael Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut Vidyasagar Dharmapuri Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut https://doi.org/10.1007/s004310000581 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004310000581 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zagariya Alexander Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut NATIONALLICENCE 700 1- Bhat Rama Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut NATIONALLICENCE 700 1- Uhal Bruce Cardiovascular Institute, Michael Reese Hospital, Chicago, IL 60616, USA, US aut NATIONALLICENCE 700 1- Navale Shankararao Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut NATIONALLICENCE 700 1- Freidine Michael Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut NATIONALLICENCE 700 1- Vidyasagar Dharmapuri Neonatology Research Laboratories, Department of Pediatrics, Michael Reese Hospital, Chicago, IL 60616, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer