caa a22 4500 475787323 CHVBK 20180406123702.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004140000151 doi (NATIONALLICENCE)springer-10.1007/s004140000151 Development of haemoglobin subtypes and extramedullary haematopoiesis in young rats. Effects of hypercapnic and hypoxic environment [Elektronische Daten] [A. Kouno, H. Inoue, T. Bajanowski, Y. Maeno, M. Iwasa, M. Nakayama, K. Nishi, B. Brinkmann, R. Matoba] The influence of repeated hypoxia on the development of haemoglobin (Hb) subtypes and on extramedullary haematopoiesis (EMH) was investigated in young Wistar rats of different ages. The rats were exposed to hypercapnic/hypoxic and to "simple” hypoxic conditions. The results obtained were compared to those of an untreated age-matched control group. Different globin chains were measured using HPLC and time-of-flight (TOF) mass analysis. The number of EMH cells was evaluated by cell counting. By determining the proportions of α- and β-chains, fetal, neonatal and mature types of globin chain composition could be differentiated. The β-2 chain levels were significantly higher in hypercapnic/hypoxic environments than in the controls and simple hypoxic environments. The numbers of EMH cells in the two groups subjected to hypercapnia/hypoxia decreased significantly more slowly compared to the controls and simple hypoxia groups. Therefore, the development of Hb subtypes and the EMH activity in rats were influenced by both repeated hypercapnia and hypoxia. Springer-Verlag Berlin Heidelberg, 2000 Key words Sudden infant death syndrome (SIDS) nationallicence Fetal nationallicence haemoglobin nationallicence Rat globin nationallicence High performance liquid chromatography (HPLC) nationallicence Extramedullary haematopoiesis (EMH) nationallicence Kouno A. Department of Legal Medicine, Osaka University School of Medicine, Yamada-oka 2-2, Suita-Osaka 565-0871, Japan, JP aut Inoue H. National Research Institute of Police Science, Kashiwa-Chiba 277-0882, Japan, JP aut Bajanowski T. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48149 Münster, Germany, DE aut Maeno Y. Department of Legal Medicine, Nagoya-City University Medical School, Kawasumi Mizuho-Nagoya 467-8601, Japan, JP aut Iwasa M. Department of Legal Medicine, Nagoya-City University Medical School, Kawasumi Mizuho-Nagoya 467-8601, Japan, JP aut Nakayama M. Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi-Osaka 594-1101, Japan, JP aut Nishi K. Department of Legal Medicine, Shiga University of Medical Science, Ohtsu-Shiga 520-2192, Japan, JP aut Brinkmann B. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48149 Münster, Germany, DE aut Matoba R. Department of Legal Medicine, Osaka University School of Medicine, Yamada-oka 2-2, Suita-Osaka 565-0871, Japan, JP aut https://doi.org/10.1007/s004140000151 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140000151 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kouno A. Department of Legal Medicine, Osaka University School of Medicine, Yamada-oka 2-2, Suita-Osaka 565-0871, Japan, JP aut NATIONALLICENCE 700 1- Inoue H. National Research Institute of Police Science, Kashiwa-Chiba 277-0882, Japan, JP aut NATIONALLICENCE 700 1- Bajanowski T. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48149 Münster, Germany, DE aut NATIONALLICENCE 700 1- Maeno Y. Department of Legal Medicine, Nagoya-City University Medical School, Kawasumi Mizuho-Nagoya 467-8601, Japan, JP aut NATIONALLICENCE 700 1- Iwasa M. Department of Legal Medicine, Nagoya-City University Medical School, Kawasumi Mizuho-Nagoya 467-8601, Japan, JP aut NATIONALLICENCE 700 1- Nakayama M. Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi-Osaka 594-1101, Japan, JP aut NATIONALLICENCE 700 1- Nishi K. Department of Legal Medicine, Shiga University of Medical Science, Ohtsu-Shiga 520-2192, Japan, JP aut NATIONALLICENCE 700 1- Brinkmann B. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48149 Münster, Germany, DE aut NATIONALLICENCE 700 1- Matoba R. Department of Legal Medicine, Osaka University School of Medicine, Yamada-oka 2-2, Suita-Osaka 565-0871, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer