caa a22 4500 475787382 CHVBK 20180406123702.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004140000140 doi (NATIONALLICENCE)springer-10.1007/s004140000140 Demonstration of myocardial necrosis in the presence of advanced putrefaction [Elektronische Daten] [C. Ortmann, H. Pfeiffer, B. Brinkmann] Samples of heart tissue were investigated in two series for the detectability of myocardial necrosis after artificial and natural putrefaction, respectively. In the first series heart tissue with and without infarction was artificially subjected to humid and dry autolysis and putrefaction. In the second investigation heart tissue was obtained from exhumed bodies after periods of burial ranging between 10 and 929 days. Besides histology a variety of immunohistochemical markers were applied and C5b-9 gave positive results even after long periods of artificial and natural putrefaction. From the methods tested, this was by far the most sensitive method with a high robustness against putrefaction. NP57, which indicates neutrophilic leucocytes could be demonstrated considerably longer after humid putrefaction than after dry putrefaction. The time limits of detection were considerably longer than for H & E. These two methods are the methods of choice for the detection of myocardial infarction and leucocyte infiltration in advanced stages of putrefaction. Springer-Verlag Berlin Heidelberg, 2000 Key words C5b-9 nationallicence NP57 nationallicence Putrefaction nationallicence Autolysis nationallicence Myocardial infarction nationallicence Ortmann C. Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48129 Münster, Germany Fax: +49-251-8355158, DE aut Pfeiffer H. Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48129 Münster, Germany Fax: +49-251-8355158, DE aut Brinkmann B. Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48129 Münster, Germany Fax: +49-251-8355158, DE aut https://doi.org/10.1007/s004140000140 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140000140 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortmann C. Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48129 Münster, Germany Fax: +49-251-8355158, DE aut NATIONALLICENCE 700 1- Pfeiffer H. Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48129 Münster, Germany Fax: +49-251-8355158, DE aut NATIONALLICENCE 700 1- Brinkmann B. Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Von-Esmarch-Strasse 62, 48129 Münster, Germany Fax: +49-251-8355158, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer