caa a22 4500 475787447 CHVBK 20180406123703.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004149900106 doi (NATIONALLICENCE)springer-10.1007/s004149900106 Genetic diversity of nine STRs in two northwest Iberian populations: Galicia and northern Portugal [Elektronische Daten] [L. Gusmão, P. Sánchez-Diz, C. Alves, M. V. Lareu, A. Carracedo, A. Amorim] The genotyping of two population samples from Galicia and northern Portugal was performed for nine STR loci using a single multiplex reaction with the AmpFlSTR Profiler Plus PCR amplification kit which co-amplifies the systems D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820 and the X-Y homologous gene amelogenin. Allele frequencies for these nine tetranucleotide repeat markers were calculated and no significant differences were observed when comparing these two populations. Conformity with Hardy-Weinberg equilibrium proportions was good for all systems in both samples. The combined power of exclusion was 99.981% and 99.980% in Galicia and northern Portugal, respectively and the combined power of discrimination was greater than 99.999%. Segregation analysis of all loci detected two incompatibilities, one in D3S1358 (out of 112 meioses) and another in D7S820 (out of 104 meioses). Both could be explained by single-step mutations. In general co-amplification was good except for some relatively degraded samples in which poor amplification was observed for the largest STRs. Nevertheless the system is technically robust even when small amounts of template DNA are used and in addition is highly informative and time-saving. However, caution should be taken in the interpretation of profiles in degraded samples and the apparently high mutation rate of D3S1358 and D7S820 should also be kept in mind. Springer-Verlag Berlin Heidelberg, 2000 Key words DNA polymorphisms nationallicence STRs nationallicence Population studies nationallicence Profiler plus nationallicence Gusmão L. IPATIMUP, Instituto de Patologia e Imunologia da Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal e-mail: aamorim@ipatimup.pt, Tel.: +351-2-5570700, Fax: +351-2-5570799, PT aut Sánchez-Diz P. Institute of Legal Medicine, University of Santiago de Compostela, 15705 Santiago de Compostela, Galicia, Spain, ES aut Alves C. IPATIMUP, Instituto de Patologia e Imunologia da Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal e-mail: aamorim@ipatimup.pt, Tel.: +351-2-5570700, Fax: +351-2-5570799, PT aut Lareu M. V. Institute of Legal Medicine, University of Santiago de Compostela, 15705 Santiago de Compostela, Galicia, Spain, ES aut Carracedo A. Institute of Legal Medicine, University of Santiago de Compostela, 15705 Santiago de Compostela, Galicia, Spain, ES aut Amorim A. IPATIMUP, Instituto de Patologia e Imunologia da Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal e-mail: aamorim@ipatimup.pt, Tel.: +351-2-5570700, Fax: +351-2-5570799, PT aut https://doi.org/10.1007/s004149900106 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004149900106 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gusmão L. IPATIMUP, Instituto de Patologia e Imunologia da Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal e-mail: aamorim@ipatimup.pt, Tel.: +351-2-5570700, Fax: +351-2-5570799, PT aut NATIONALLICENCE 700 1- Sánchez-Diz P. Institute of Legal Medicine, University of Santiago de Compostela, 15705 Santiago de Compostela, Galicia, Spain, ES aut NATIONALLICENCE 700 1- Alves C. IPATIMUP, Instituto de Patologia e Imunologia da Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal e-mail: aamorim@ipatimup.pt, Tel.: +351-2-5570700, Fax: +351-2-5570799, PT aut NATIONALLICENCE 700 1- Lareu M. V. Institute of Legal Medicine, University of Santiago de Compostela, 15705 Santiago de Compostela, Galicia, Spain, ES aut NATIONALLICENCE 700 1- Carracedo A. Institute of Legal Medicine, University of Santiago de Compostela, 15705 Santiago de Compostela, Galicia, Spain, ES aut NATIONALLICENCE 700 1- Amorim A. IPATIMUP, Instituto de Patologia e Imunologia da Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal e-mail: aamorim@ipatimup.pt, Tel.: +351-2-5570700, Fax: +351-2-5570799, PT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer