caa a22 4500 475787463 CHVBK 20180406123703.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004140000161 doi (NATIONALLICENCE)springer-10.1007/s004140000161 A new pentaplex PCR system for forensic casework analysis [Elektronische Daten] [T. Lederer, S. Seidl, B. Graham, P. Betz] In 1998 the Federal Criminal Police Office of Germany (BKA) established a central genetic database of offenders and suspects to facilitate comparisons with biological samples from future criminal offences. The five obligatory short tandem repeat (STR) loci in this database (TH01, SE33, vWA, FGA and D21S11) were co-amplified in a new PCR pentaplex analysing system together with the sex-specific locus amelogenin. Due to overlapping fragment sizes, amplification products were fluorescent dye-labelled with different colours, separated by electrophoresis and detected directly using the ABI PRISM 310 Genetic Analyzer. Reproducible and reliable results were obtained from as low as 125 pg template DNA, indicating high specificity and sensitivity of the assay. Environmental studies and enzymatic digest with DNase I revealed an excellent stability of the pentaplex system with typeable results even in cases of partially degraded DNA. Complete and reproducible DNA typing was possible in bloodstain mixtures with the minor component as low as 10%. Mean stutter peak intensities were analysed for all loci and ranged from 2.7 ± 0.8% (TH01) to 10.6 ± 1.6% (vWA) of the main signal intensity. Allele frequencies were determined in a North Bavarian population sample (n = 121). The combination of five systems resulted in a mean exclusion chance of 99.86% and a power of discrimination of 99.999996%. No deviation from Hardy-Weinberg equilibrium could be found. Springer-Verlag Berlin Heidelberg, 2000 Key words Pentaplex nationallicence Multiplex nationallicence STR nationallicence DNA typing nationallicence Lederer T. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut Seidl S. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut Graham B. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut Betz P. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut https://doi.org/10.1007/s004140000161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140000161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lederer T. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut NATIONALLICENCE 700 1- Seidl S. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut NATIONALLICENCE 700 1- Graham B. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut NATIONALLICENCE 700 1- Betz P. Institute for Forensic Medicine, University Erlangen-Nürnberg, Universitätsstrasse 22, 91054 Erlangen, Germany e-mail: thomas.lederer@rzmail.uni-erlangen.de; Tel.: +49-9131-8522272; Fax: +49-9131-8522274, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer